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Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF

Catalog #: 8989-T2
1 Citation 2 Reviews Datasheet / COA / SDS
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8989-T2-050
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Citations (1)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LILRB1/CD85j/ILT2 is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED50 of 0.02-0.2 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human LILRB1/CD85j/ILT2 protein
Human LILRB1
(Gly24-His458)
Accession # Q8NHL6		HHHHHH
N-terminusC-terminus
Accession #
Q8NHL6
N-terminal Sequence
Analysis
Gly24
Structure / Form
Monomer
Predicted Molecular Mass
48 kDa
SDS-PAGE
65-82 kDa, reducing conditions

Product Datasheets

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8989-T2

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8989-T2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human LILRB1/CD85j/ILT2 His-tag (Catalog # 8989-T2) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (914-AN) binds with a typical ED50 of 0.02-0.2 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRB1/CD85j/ILT2

LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family (1). Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs (2). Alternative splicing generates an additional isoform that lacks the transmembrane and cytoplasmic regions (3). LILRB1 is expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma δ T cells, and monocyte-derived dendritic cells (3-7). LILRB1 binds to MHC-I as well as non-classical MHC-I molecules (e.g. HLA-F, HLA-G, and HLA-B27) and the MHC-I mimetic UL18 encoded by cytomegalovirus (3, 5, 8-10). R&D Systems
in-house testing indicates that LILRB1 also binds to Angiopoietin-like 7. Ligation of LILRB1 inhibits the antigen induced proliferation and activation of CD8+ T cells, CD4+ T cells, NK cells, and gamma δ T cells (3, 4, 11-13). On dendritic cells, ligation inhibits the production of IL-10, IL-12p70, and TGF-beta and protects from Fas-mediated apoptosis (7).

References
  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Samaridis, J. and M. Colonna (1997) Eur. J. Immunol. 27:660.
  3. Colonna, M. et al. (1997) J. Exp. Med. 186:1809.
  4. Harly, C. et al. (2011) Blood 117:2864.
  5. Cosman, D. et al. (1997) Immunity 7:273.
  6. Young, N.T. et al. (2001) J. Immunol. 166:3933.
  7. Young, N.T. et al. (2008) Blood 111:3090.
  8. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552.
  9. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856.
  10. Allen, R.L. et al. (2001) J. Immunol. 167:5543.
  11. Ince, M.N. et al. (2004) Immunology 112:531.
  12. Saverino, D. et al. (2000) J. Immunol. 165:3742.
  13. Saverino, D. et al. (2002) J. Immunol. 168:207.
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 1
Entrez Gene IDs
10859 (Human)
Alternate Names
CD85 antigen-like family member J; CD85j antigen; CD85j; Ig-like transcript 2; ILT2; ILT-2; ILT2FLJ37515; Immunoglobulin-like transcript 2; leukocyte Ig-like receptor-1; Leukocyte immunoglobulin-like receptor 1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB1; LIR1; LIR1LIR-1MIR-7CD85MIR7CD85 antigen; member 1; MIR7; Monocyte/macrophage immunoglobulin-like receptor 7

Citation for Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Macrophage Biomarkers sCD163 and sSIRPalpha in Serum Predict Mortality in Sarcoma Patients
    Authors: N Aggerholm-, HN Friis, T Baad-Hanse, HJ Møller, B Sandfeld-P
    Cancers, 2023-02-28;15(5):.
    Species: Human
    Sample Types: Serum
    Applications: Reference Standard

FAQs

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Reviews for Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF
By Anonymous on 09/01/2021
Application: Binding assay/Protein-protein interaction
Reason for Rating: Confirmed binding of ILT2 and HLA-G reagent. Binding is weak so not able to obtain affinity and kinetic measurements. But did see specific binding to ILT2 compared with control

immobilize HLA-G and flow ILT2-his

Binding assay/Protein-protein interaction Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF 8989-T2
Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF
By Anonymous on 05/06/2020
Application: Binding assay/Protein-protein interaction
Reason for Rating: good material

Performed binding specificity experiments for the lead molecule (xLILRA6) on its ligand family panels (RA1, RA2, RA5, RA6, RB1, RB2, RB3, RB4, RB5)

Binding assay/Protein-protein interaction Recombinant Human LILRB1/CD85j/ILT2 His-tag Protein, CF 8989-T2