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Recombinant Human LRRN2 His-tag, CF

Catalog #: 10228-LR Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10228-LR-050
Recombinant Human LRRN2 His-tag SDS-PAGE
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LRRN2 His-tag, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human LRRN2, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Source
Chinese Hamster Ovary cell line, CHO-derived human LRRN2 protein
Ala19-Gly630, with a C-terminal 6-His tag
Accession #
O75325
N-terminal Sequence
Analysis
Ala19 & Val20
Predicted Molecular Mass
69 kDa
SDS-PAGE
73-81 kDa, under reducing conditions

Product Datasheets

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10228-LR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10228-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE Recombinant Human LRRN2 His-tag SDS-PAGE View Larger

2 μg/lane of Recombinant Human LRRN2 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-81 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRRN2

Leucine-rich repeat neuronal protein 2 (LRRN2) is a type I transmembrane protein containing leucine-rich repeats (1). Mature human LRRN-2 consists of a 612 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 62 aa cytoplasmic domain. Within the ECD, human LRRN-2 shares 92% with mouse and rat LRRN-2. LRRN-2 is expressed in neuronal tissue postsynaptic membranes (1, 2). It is involved in the development and maintenance of excitatory synapse in the vertebrate nervous system. LRRN-2 regulates surface expression of AMPA receptors and instructs the development of functional glutamate release sites (1, 2). LRRN-2 is up-regulated after NGF-induced differentiation and down-regulated after NGF depletion-induced apoptosis in mouse superior cervical ganglion cells (2).

References
  1. Lauren, J. et al. (2003) Genomics 81:411.
  2. Hamano, S. et al. (2004) Int. J. Oncol. 24:1457.
Long Name
Leucine-rich Repeat Neuronal 2
Entrez Gene IDs
10446 (Human); 16980 (Mouse); 289020 (Rat)
Alternate Names
FIGLER7; GAC1; GAC1LRRN5; Glioma amplified on chromosome 1 protein; immunoglobulin and leucine rich repeat domain 7; leucine rich and ankyrin repeats 1; leucine rich repeat neuronal 2; leucine rich repeat neuronal 5; leucine-rich repeat neuronal protein 2; Leucine-rich repeat neuronal protein 5; LRANK1; LRRN2; LRRN5

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