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Recombinant Human/Mouse/Rat UbcH7/UBE2L3 Protein, CF

Catalog #: E2-640
21 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
E2-640-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (21)
FAQs
Reviews (3)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human/Mouse/Rat UbcH7/UBE2L3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human UbcH7/UBE2L3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH7/UBE2L3 concentration of 0.1-1 μM.
Source
E. coli-derived UbcH7/UBE2L3 protein
Met1 - Met154
Accession #
P68036.1
Predicted Molecular Mass
18 kDa

Product Datasheets

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E2-640

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-640

Formulation Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UbcH7/UBE2L3

Ubiquitin-conjugating Enzyme H7 (UbcH7), also known as Ubiquitin-conjugating Enzyme E2L 3 (UBE2L3), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). It has a predicted molecular weight of approximately 18 kDa. The human UbcH7 protein shares 100% amino acid (aa) sequence identity with the mouse and rat orthologs. UbcH7 has an E2 catalytic core domain that contains an active site cysteine residue and comprises 152 of its 154 aa residues. UbcH7 is catalytically active with HECT and RBR domain-containing families of Ubiquitin ligases (E3s) (2,3). UbcH7 localizes to both the nucleus and cytoplasm in human cells. In mice, its ortholog is expressed in many tissues including brain, muscle, heart, lung, lymph node, spleen, thymus, and testis (4,5). UbcH7 depletion results in an extended S phase and a reduced rate of proliferation, suggesting that it may play a role in the cell cycle (6). In humans, single nucleotide polymorphisms in UbcH7 are associated with systemic lupus erythematosus and Crohn's disease, suggesting that UbcH7 is important for proper immune system function (7,8).

 

References
  1. Nuber, U. et al. (1996) J. Biol. Chem. 271:2795.
  2. Huang, L. et al. (1999) Science 286:1321.
  3. Wenzel, D.M. et al. (2011) Nature 474:105.
  4. Garside, H. et al. (2006) J. Endocrinol. 190:621.
  5. Harbers, K. et al. (1996) Proc. Natl. Acad. Sci. USA 93:12412.
  6. Whitcomb, E.A. et al. (2009) Mol. Biol. Cell 20:1.
  7. Wang, S. et al. (2012) Genes Immun. 13:380.
  8. Fransen, K. et al. (2010) Hum. Mol. Genet. 19:3482.
 
Entrez Gene IDs
7332 (Human); 22195 (Mouse); 363836 (Rat)
Alternate Names
E2-F1; EC 6.3.2.19; L-UBC; UBCE7; UbcH7; UBCH7UbcM4; UbcM4; UBE2L3; Ubiquitin carrier protein L3; ubiquitin-conjugating enzyme E2 L3; Ubiquitin-conjugating enzyme E2-F1; ubiquitin-conjugating enzyme E2L 3; ubiquitin-conjugating enzyme UBCH7; Ubiquitin-protein ligase L3

Citations for Recombinant Human/Mouse/Rat UbcH7/UBE2L3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Selective Smurf1 E3 ligase inhibitors that prevent transthiolation
    Authors: Chuong, P;Statsyuk, A;
    bioRxiv : the preprint server for biology
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Parkin Precipitates on Mitochondria via Aggregation and Autoubiquitination
    Authors: Ardah, MT;Radwan, N;Khan, E;Kitada, T;Haque, ME;
    International journal of molecular sciences
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma
    Authors: X Du, J Pang, B Gu, T Si, Y Chang, T Li, M Wu, Z Wang, Y Wang, J Feng, N Wu, J Man, H Li, A Li, T Zhang, B Wang, X Duan
    Nucleic Acids Research, 2023-02-22;0(0):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. OTUB1 suppresses Hippo signaling via modulating YAP protein in gastric cancer
    Authors: C Yan, H Yang, P Su, X Li, Z Li, D Wang, Y Zang, T Wang, Z Liu, Z Bao, S Dong, T Zhuang, J Zhu, Y Ding
    Oncogene, 2022-10-21;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease
    Authors: SK Pirooznia, H Wang, N Panicker, M Kumar, S Neifert, MA Dar, E Lau, BG Kang, J Redding-Oc, JC Troncoso, VL Dawson, TM Dawson
    Science Advances, 2022-04-01;8(13):eabh1824.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes
    Authors: S Yoon, K Bogdanov, D Wallach
    Cell Death and Differentiation, 2022-01-09;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  7. Identification of disease-linked hyperactivating mutations in UBE3A through large-scale functional variant analysis
    Authors: KP Weston, X Gao, J Zhao, KS Kim, SE Maloney, J Gotoff, S Parikh, YC Leu, KP Wu, M Shinawi, JP Steimel, JS Harrison, JJ Yi
    Nature Communications, 2021-11-23;12(1):6809.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  8. Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection
    Authors: EG Otten, E Werner, A Crespillo-, KB Boyle, V Dharamdasa, C Pathe, B Santhanam, F Randow
    Nature, 2021-05-19;0(0):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  9. CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage
    Authors: MC Albert, K Brinkmann, W Pokrzywa, SD Günther, M Krönke, T Hoppe, H Kashkar
    Cell Death & Disease, 2020-09-10;11(9):740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitylation
  10. The Ubiquitin Ligase TRIP12 Limits PARP1 Trapping and Constrains PARP Inhibitor Efficiency
    Authors: M Gatti, R Imhof, Q Huang, M Baudis, M Altmeyer
    Cell Rep, 2020-08-04;32(5):107985.
    Species: Human
    Sample Types: Protein, Recombinant Protein
    Applications: Bioassay
  11. K27-linked ubiquitination of BRAF by ITCH engages cytokine response to maintain MEK-ERK signaling
    Authors: Q Yin, T Han, B Fang, G Zhang, C Zhang, ER Roberts, V Izumi, M Zheng, S Jiang, X Yin, M Kim, J Cai, EB Haura, JM Koomen, KSM Smalley, L Wan
    Nat Commun, 2019-04-23;10(1):1870.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  12. Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability
    Authors: M Bosshard, R Aprigliano, C Gattiker, V Palibrk, E Markkanen, P Hoff Backe, S Pellegrino, FL Raymond, G Froyen, M Altmeyer, M Bjørås, GL Dianov, B van Loon
    Sci Rep, 2017-11-08;7(1):15050.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  13. Mechanism of Ubiquitin Chain Synthesis Employed by a HECT Ubiquitin Ligase
    Authors: ME French, JL Klosowiak, A Aslanian, SI Reed, JR Yates, T Hunter
    J. Biol. Chem., 2017-05-01;0(0):.
    Applications: Bioassay
  14. H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis
    Authors: X Zhang, B Li, AH Rezaeian, X Xu, PC Chou, G Jin, F Han, BS Pan, CY Wang, J Long, A Zhang, CY Huang, FJ Tsai, CH Tsai, C Logothetis, HK Lin
    Nat Commun, 2017-03-16;8(0):14799.
    Applications: Bioassay
  15. The Nedd8 Non-covalent Binding Region in the Smurf HECT Domain is Critical to its Ubiquitn Ligase Function
    Authors: S He, Y Cao, P Xie, G Dong, L Zhang
    Sci Rep, 2017-02-07;7(0):41364.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  16. Monoubiquitination Inhibits the Actin Bundling Activity of Fascin
    Authors: Shengyu Yang
    J. Biol. Chem., 2016-11-22;0(0):.
    Applications: Bioassay
  17. Smurf2 negatively modulates RIG-I-dependent antiviral response by targeting VISA/MAVS for ubiquitination and degradation.
    Authors: Pan Y, Li R, Meng J, Mao H, Zhang Y, Zhang J
    J Immunol, 2014-04-11;192(10):4758-64.
    Species: Human
    Sample Types: Whole Cells
  18. Ubiquitination increases parkin activity to promote autophagic alpha-synuclein clearance.
    Authors: Lonskaya I, Desforges N, Hebron M, Moussa C
    PLoS ONE, 2013-12-26;8(12):e83914.
    Applications: Enzyme Assay
  19. Parkin ubiquitinates Tar-DNA binding protein-43 (TDP-43) and promotes its cytosolic accumulation via interaction with histone deacetylase 6 (HDAC6).
    Authors: Hebron, Michaeli, Lonskaya, Irina, Sharpe, Kaydee, Weerasinghe, Puwakdan, Algarzae, Norah K, Shekoyan, Ashot R, Moussa, Charbel
    J Biol Chem, 2012-12-20;288(6):4103-15.
    Applications: Bioassay
  20. Characterization of ML-IAP protein stability and physiological role in vivo.
    Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj
    Biochem J, 2012-11-01;447(3):427-36.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  21. ERalpha phosphorylation at Y537 by Src triggers E6-AP-ERalpha binding, ERalpha ubiquitylation, promoter occupancy, and target gene expression.
    Authors: Sun J, Zhou W, Kaliappan K, Nawaz Z, Slingerland J
    Mol Endocrinol, 2012-08-03;26(9):1567-77.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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Reviews for Recombinant Human/Mouse/Rat UbcH7/UBE2L3 Protein, CF

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Recombinant Human UbcH7/UBE2L3 Protein, CF
By Patrick Chuong on 01/27/2020
Application: Enzymatic activity in vitro
Enzymatic activity in vitro Recombinant Human UbcH7/UBE2L3 Protein, CF E2-640
Recombinant Human UbcH7/UBE2L3 Protein, CF
By Patrick Chuong on 08/05/2019
Application: Enzymatic activity in vitro
Enzymatic activity in vitro Recombinant Human UbcH7/UBE2L3 Protein, CF E2-640
Recombinant Human UbcH7/UBE2L3 Protein, CF
By Liping Yang on 05/18/2016
Application: In vitro bioactivity in cell culture
Reason for Rating: I try to find the specific E3 ligase of my substrate from cell lysate, this E2 is very good for this experiment.

From the attached image, the first lane is reaction with this product, while the second lane is without it. We can see the difference between the ubiquitination smears is very significant.

In vitro bioactivity in cell culture Recombinant Human UbcH7/UBE2L3 Protein, CF E2-640