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Recombinant Human Nidogen-1/Entactin Protein, CF

Catalog #: 2570-ND
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2570-ND-050
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Citations (7)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Nidogen-1/Entactin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 4 x 104 cells/well are added to rhNidogen-1 coated plates (30 µg/mL with 100 µL/well), approximately 40-75% will adhere after one hour at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human Nidogen-1/Entactin protein
Leu29-Lys1114 (Gln1113Arg), with an N-terminal 9-His tag
Accession #
AAH45606
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
120 kDa
SDS-PAGE
130 kDa, reducing conditions

Product Datasheets

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2570-ND

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2570-ND

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nidogen-1/Entactin

Nidogen-1 (also entactin) is a 150 kDa, secreted, monomeric glycoprotein that serves as a major linking component of basement membranes (1 - 4). It is synthesized as a 1247 amino acid (aa) precursor with a 28 aa signal sequence and a 1219 aa mature protein. The molecule is modular in structure with five distinct regions. There are three globular domains (G1-3) separated by a mucin region and an extended rod-shaped segment (5 - 7). The N-terminal globular domain (G1) is 200 aa in length and seemingly unrelated to any known motif (8). The mucin region is nearly 160 aa in length and presumably O-glycosylated (2, 8). G2 and G3 are both approximately 300 aa in length. G2 is described as a Nidogen ( beta -barrel) domain, while C-terminal G3 assumes a beta -propeller configuration (1). The 250 aa rod-shaped segment has multiple EGF-like motifs and two thyroglobulin type 1 domains. Functionally, G1 is reported to bind type IV collagen (2, 7). The mucin region contains a short peptide that ligates alpha 3 beta 1 integrins (9, 10). G2 interacts with perlecan, and an RGD motif in the rod-shaped segment serves as a binding site for alpha v beta 3 integrins (9, 10). Finally, G3 is associated with laminin binding (2, 7). As a full-length molecule, the multiple extracellular matrix-binding sites of Nidogen-1 are well positioned to serve as anchor sites for basement membrane molecules. Nidogen-1 also undergoes proteolytic processing by at least two MMPs, MMP7 and MMP19 (10, 11). While this destroys the integrity of Nidogen-associated matrices, it also generates peptide fragments that are capable of inducing neutrophil chemotaxis and phagocytosis (10). Nidogen-2 is related to Nidogen-1 (≈ 50% aa identity) and shares many of the same adhesive properties as Nidogen-1 (12). Both bind perlecan plus collagens I and IV. Nidogen-2, however, does not bind fibulin-1 or 2, and shows only modest interaction with laminin. Thus, although coexpressed, Nidogen-2 serves as only a partial substitute for Nidogen-1 (2, 12). Human Nidogen-1 is 85% aa identical to both mouse and rat Nidogen-1, and 88% aa identical to canine Nidogen-1.

References
  1. Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115. 
  2. Miosge, N. et al. (2001) Histochem. J. 33:523.
  3. Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
  4. Timpl, R. and J.C. Brown (1996) BioEssays 18:123. 
  5. Nagayoshi, T. et al. (1989) DNA 8:581. 
  6. Zimmerman, K. et al. (1995) Genomics 27:245. 
  7. Fox, J.W. et al. (1991) EMBO J. 10:3137.
  8. Mayer, U. et al. (1995) Eur. J. Biochem. 227:681.
  9. Gresham, H.D. et al. (1996) J. Biol. Chem. 271:30587.
  10. Dong, L-J. et al. (1995) J. Biol. Chem. 270:15383.
  11. Titz, B. et al. (2004) Cell. Mol. Life Sci. 61:1826.
  12. Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.
Entrez Gene IDs
4811 (Human)
Alternate Names
enactin; Entactin; Entactin-1; NID1; NID-1; NIDentactin; nidogen (enactin); nidogen 1; Nidogen1; Nidogen-1

Citations for Recombinant Human Nidogen-1/Entactin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE
    Authors: T Viheriälä, J Sorvari, TO Ihalainen, A Mörö, P Grönroos, S Schlie-Wol, B Chichkov, H Skottman, S Nymark, T Ilmarinen
    Scientific Reports, 2021-01-13;11(1):933.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Endothelial cell-derived nidogen-1 inhibits migration of SK-BR-3 breast cancer cells
    Authors: DA Ferraro, F Patella, S Zanivan, C Donato, N Aceto, M Giannotta, E Dejana, M Diepenbruc, G Christofor, M Buess
    BMC Cancer, 2019-04-04;19(1):312.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Nidogen-1 Degraded by Cathepsin S can be Quantified in Serum and is Associated with Non-Small Cell Lung Cancer
    Authors: N Willumsen, CL Bager, DJ Leeming, AC Bay-Jensen, MA Karsdal
    Neoplasia, 2017-03-07;19(4):271-278.
    Species: Human
    Sample Types: Protein
    Applications: Enzyme Assay
  4. The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet's Membrane Components
    Invest Ophthalmol Vis Sci, 2016-09-01;57(11):4599-605.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Proteolytic activity of prostate-specific antigen (PSA) towards protein substrates and effect of peptides stimulating PSA activity.
    Authors: Mattsson J, Ravela S, Hekim C, Jonsson M, Malm J, Narvanen A, Stenman U, Koistinen H
    PLoS ONE, 2014-09-19;9(9):e107819.
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: Bioassay, Enzyme Assay
  6. SgrA, a nidogen-binding LPXTG surface adhesin implicated in biofilm formation, and EcbA, a collagen binding MSCRAMM, are two novel adhesins of hospital-acquired Enterococcus faecium.
    Authors: Hendrickx AP, van Luit-Asbroek M, Schapendonk CM, van Wamel WJ, Braat JC, Wijnands LM, Bonten MJ, Willems RJ
    Infect. Immun., 2009-09-08;77(11):5097-106.
    Species: Bacteria
    Sample Types: Bacteria
    Applications: Binding Assay
  7. The impact of extracellular matrix coatings on the performance of human renal cells applied in bioartificial kidneys.
    Authors: Zhang H, Tasnim F, Ying JY, Zink D
    Biomaterials, 2009-02-12;30(15):2899-911.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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