Recombinant Human NKp80/KLRF1 Fc Chimera Protein, CF Summary
Product Specifications
| MD | Human IgG1 (Pro100 - Lys330) |
IEGR | Human NKp80 (Val66 - Tyr231) Accession # Q9NZS2 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1900-NK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: NKp80/KLRF1
NKp80, also known as killer cell lectin-like receptor subfamily F member 1 (KLRF1), is an 80 kDa type II transmembrane, homodimeric, activating coreceptor expressed exclusively on the surface of NK cells. Human NKp80, a member of the C-type lectin family of proteins (1 - 3), is 231 aa in length with a 38 amino acid (aa) cytoplasmic region, a 21 aa transmembrane region, and a 172 aa extracellular domain (ECD) (SwissProt # Q9NZS2). The ECD contains the C-type lectin-like domain (aa 114 - 230) and four potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for NKp80. Isoform 1 is the standard protein. There are no murine orthologs for NKp80 (4). One early study suggested that NKp80 functions as an inhibitory receptor based on the presence of two immunoreceptor tyrosine-based inhibitory motifs (ITIM) in the N-terminal cytoplasmic region, and the lack of charged amino acids in the transmembrane region (1). However, later studies show that NKp80 displays an activating function rather than an inhibitory one (2 - 3, 5). In in vitro experiments in human NK cells, NKp80 stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies (2 - 3). One study reports that NKp80 likely functions as a coreceptor, cooperating with NKp46 and other activating receptors in NK cell cytotoxicity (2). Activation-induced C-type lectin (AICL) is the ligand for NKp80 (3). It is a myeloid-specific receptor expressed by monocytes, macrophages, and granulocytes (3). Crosslinking of both NKp80 and AICL stimulates secretion of proinflammatory cytokines suggesting that NKp80-AICL interaction is involved in the activating crosstalk between NK cells and myeloid cells, and thus may influence the initiation and maintenance of immune responses in humans (3).
- Roda-Navarro, P. et al. (2000) Eur. J. Immunol. 30:568.
- Vitale, M. et al. (2001) Eur. J. Immunol. 31:233.
- Welte, S. et al. (2006) Nat. Immunol. 7:1334.
- Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
- Thomas, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:1656.
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