Recombinant Human NRG1 isoform 9/GGF-2 Protein, CF New
Recombinant Human NRG1 isoform 9/GGF-2 Protein, CF Summary
Product Specifications
The ED50 for this effect is 0.750-7.50 ng/mL.
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
11505-NR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Scientific Data
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Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 0.750-7.50 ng/mL.
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2 μg/lane of Recombinant Human NRG1 isoform 9/GGF-2 Protein (Catalog # 11505-NR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70‑80 kDa, under reducing conditions.
Reconstitution Calculator
Background: Neuregulin-1/NRG1
Glial growth factor2, GGF-2, also known as cimaglermin alfa or NRG1 beta 3 (1), is a full-length splice variant of the Neuregulin-1 (NRG1) family gene. NRG1 is one of the four members of the neuregulin family of genes (NRG1-4) encoding signaling proteins that facilitate cell–cell interactions in many tissues, including the nervous system and the heart (2). All family members share an EGF-like domain that interacts with the ErbB family of tyrosine kinase receptors. Through their displayed interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells. The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.Neuregulins are emerging as potential treatments for heart failure. GGF-2 was chosen as a development candidate with potentially long-lasting pharmacodynamic activity (4).
- Dimayuga, F.O. et al. (2003) J Neuroimmunol 136:67.
- Falls D.L. (2003). Exp. Cell Res. 284:14.
- Marchionni M.A. et al. (1999) Adv Exp Med Biol. 468:283.
- Caggiano A.O. et al. (2017) Eur J Pharmacol.796:76.
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