Recombinant Human Properdin Protein, CF

Catalog #: 8216-PR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8216-PR-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Human Properdin Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Zymosan is coated at 200 μg/mL (100 μL/well), the concentration of Recombinant Human Properdin that produces 50% optimal binding response is 0.25-1.25 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human Properdin protein
Asp28-Leu469, with a C-terminal 10-His tag

Accession #

P27918

N-terminal Sequence
Analysis

Asp28

Predicted Molecular Mass

50 kDa

SDS-PAGE

57-63 kDa, reducing conditions

Product Datasheets

8216-PR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8216-PR

Formulation	Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Properdin

Properdin, also known as Complement Factor P, is an approximately 53 kDa glycoprotein that positively regulates the complement alternative pathway (AP) (1, 2). It consists of seven thrombospondin repeat (TSR) domains, referred to as TSR0-TSR6 beginning at the N-terminus (3). Under physiological conditions, Properdin can form cyclic dimers, trimers, and tetramers through head-to-tail interactions of monomeric subunits (4-6). Non-physiological high molecular weight polymers of Properdin can also form during long term storage and freeze/thaw cycles (5, 7). Mature human Properdin shares 79% and 77% amino acid sequence identity with rat and mouse Properdin, respectively. Interestingly, human Properdin has been shown to restore LPS-dependent AP complement activity in Properdin^-/- mouse serum (8). Properdin may also act as a pattern-recognition molecule, since it binds glycosaminoglycan (GAG) chains of proteoglycans on apoptotic T cells to promote complement activation and phagocytosis (9). Furthermore, Properdin has been shown to bind certain microbial targets and serve as a platform for C3bBbP assembly and function (10, 11). Underscoring its importance to immunity, Properdin deficiency is associated with significant risk of meningococcal disease in humans (12). It may also be involved in the development of abdominal aortic aneurysm, an inflammatory vascular disease (13).

References