Recombinant Human Protocadherin-12 Protein, CF

Recombinant Human Protocadherin-12 Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Protocadherin-12

Protocadherin-12 (PCDH12; also called VE-Cadherin-2) is an approximately 150 kDa glycoprotein belonging to the δ2 subgroup of non-clustered protocadherins. These are type I transmembrane proteins that exhibit calcium-dependent but relatively weak homophilic adhesion (1-4). Like other δ2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1). Within the ECD, human Protocadherin-12 shares 83% and 82% amino acid sequence identity with mouse and rat Protocadherin-12, respectively. Protocadherin-12 is expressed in mouse endothelial,
glycogen-rich trophoblast, and mesangial cells where it clusters at intercellular junctions (1, 2). It may be important during placental development, as placentas from Protocadherin-12-deficient mice display altered morphogenesis and transcription profiles (3). In humans, the ECD of Protocadherin-12 can be shed via proteolytic cleavage (5). It circulates at increased levels in sera from pregnant women exhibiting pre-eclampsia compared to women with nonpathological gestations (5).rich

Related Research Areas

• Cadherin Superfamily
• Calcium-binding Proteins and Related Molecules