Recombinant Human ROBO2 Fc Chimera Protein, CF Summary
Product Specifications
| Human ROBO2 (Ser22-Pro863) Accession # NP_001276969 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3147-RB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: ROBO2
Human ROBO2 is a 175 kDa member of the ROBO family of guidance molecules (1-3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Human ROBO2 is a type I transmembrane (TM) glycoptotein that is synthesized as a 1378 amino acid (aa) precursor. It contains a 21 aa signal sequence, an 838 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 498 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 31-504) and three fibronectin (FN) type III domains (aa 522-826). The cytoplasmic region contains multiple 15-20 aa long CC (conserved cytoplasmic) motifs (C0-C2) that are found in ROBO-1 (7, 8). Human ROBO2 has at least two potential isoforms. One isoform shows a cytoplasmic truncation of the C-terminus (aa 1186-1378) (9). A second isoform is a 570 aa soluble form that shows a deletion of the first two and one-half C2-type Ig-like domains (aa 22 - 285) and terminates after the third fibronectin domain with a unique 20 aa sequence (10). Based on mouse and rat ROBO2 GenBank sequences, considerably more alternate splice forms may occur. Human ROBO2 ECD is 98% aa identical to the ECD in mouse and canine ROBO2. ROBO2 would appear to play a number of roles in cell adhesion. In the neural tube, it is the receptor for a chemorepulsant. Axons that cross the midline are directed, presumably by ROBO2/SLIT chemorepulsion, to lateral positions in the contralateral spinal cord (6, 11, 12). In addition, ROBO2 is both permissive for neurite outgrowth (via ROBO1‑ROBO2 interaction) and inhibitory for neurite outgrowth (via ROBO2-SLIT interaction) (13, 14).
- Rajagopalan, S. et al. (2000) Neuron 28:767.
- Guthrie, S. (2004) Curr. Biol. 14:R632.
- Guthrie, S. (2001) Curr. Biol. 11:R300.
- Seeger, M. et al. (1993) Neuron 10:409.
- Kidd, T. et al. (1998) Cell 92:205.
- Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
- Bashaw, G.J. et al. (2000) Cell 101:703.
- Jen, J.C. et al. (2004) Science 304:1509.
- GenBank Accession # Q9KCK4.
- Isogai, T. et al. (2002) GenBank Accession # AK074780.
- Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41.
- Long, H. et al. (2004) Neuron 42:213.
- Hivert, B. et al. (2002) Mol. Cell. Neurosci. 21:534.
- Lin, L. et al. (2005) Mol. Cell. Neurosci. 28:547.
Citations for Recombinant Human ROBO2 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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ROBO2 signaling in lung development regulates SOX2/SOX9 balance, branching morphogenesis and is dysregulated in nitrofen-induced congenital diaphragmatic hernia
Authors: AN Gonçalves, J Correia-Pi, C Nogueira-S
Respir Res, 2020-11-18;21(1):302.
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture -
Estrogen receptor (ER) agonists differentially regulate neuroangiogenesis in peritoneal endometriosis via the repellent factor SLIT3.
Authors: Greaves E, Collins F, Esnal-Zufiaurre A, Giakoumelou S, Horne A, Saunders P
Endocrinology, 2014-07-22;155(10):4015-26.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Slit-Robo signals regulate pioneer axon pathfinding of the tract of the postoptic commissure in the mammalian forebrain.
Authors: Ricano-Cornejo I, Altick AL, Garcia-Pena CM, Nural HF, Echevarria D, Miquelajauregui A, Mastick GS, Varela-Echavarria A
J. Neurosci. Res., 2011-06-17;89(10):1531-41.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay
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