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Recombinant Human S100A7 Protein, CF

Catalog #: 9085-SA
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9085-SA-050
Recombinant Human S100A7 Protein Bioactivity
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Product Details
Citations (1)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human S100A7 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 2-10 μg/mL.
Source
E. coli-derived human S100A7 protein
Ser2-Gln101 with an N-terminal Met
Accession #
AAH34687
N-terminal Sequence
Analysis
Ser2 (Major) & Met1 (Minor)
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
11 kDa
SDS-PAGE
11 kDa, reducing conditions

Product Datasheets

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9085-SA

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9085-SA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human S100A7 Protein Bioactivity View Larger

Recombinant Human S100A7 (Catalog # 9085-SA) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 2-10 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A7

S100A7, also known as Psoriasin, is a 11-12 kDa member of the S100 family of EF hand calcium binding proteins (1). Human S100A7 shares 32% amino acid sequence identity with mouse S100A7A, the closest related protein in mouse (2). It is acetylated at the N-terminus and binds both calcium and zinc ions (3, 4). S100A7 is up-regulated in keratinocytes of psoriasis and atopic dermatitis lesions (5-9), as well as in epithelial cells of the tongue, eye, and female genital tract (10-12). Its up-regulation can be induced by bacterial exposure, inflammatory cytokines, or epidermal barrier disruption (3, 9, 11, 12). S100A7 supports epithelial integrity through killing E. coli by sequestration of zinc (3, 5) and through inducing the up-regulation of tight junction proteins (13). The interaction of S100A7 with RAGE promotes the migration of immune cells and the infiltration of macrophages into tumor sites (7, 14).

References
  1. Donato, R. et al. (2013) Curr. Mol. Med. 13:24.
  2. Madsen, P. et al. (1991) J. Invest. Dermatol. 97:701.
  3. Glaser, R. et al. (2005) Nat. Immunol. 6:57.
  4. Brodersen, D.E. et al. (1999) Biochemistry 38:1695.
  5. Lee, K.C. and R.L. Eckert (2007) J. Invest. Dermatol. 127:945.
  6. Anderson, K.S. et al. (2009) Br. J. Dermatol. 160:325.
  7. Wolf, R. et al. (2008) J. Immunol. 181:1499.
  8. Al-Haddad, S. et al. (1999) Am. J. Pathol. 155:2057.
  9. Glaser, R. et al. (2009) J. Invest. Dermatol. 129:641.
  10. Meyer, J.E. et al. (2008) Mucosal Immunol. 1:239.
  11. Garreis, F. et al. (2011) Invest. Opthalmol. Vis. Sci. 52:4914.
  12. Mildner, M. et al. (2010) Mucosal Immunol. 3:602.
  13. Hattori, F. et al. (2014) Br. J. Dermatol. 171:742.
  14. Nasser, M.W. et al. (2015) Cancer Res. 75:974.
Long Name
S100 Calcium Binding Protein A7
Entrez Gene IDs
6278 (Human); 192344 (Mouse)
Alternate Names
HID 5; HID5; HID-5; protein S100-A7; PSOR1; psoriasin 1; Psoriasin; S100 calcium binding protein A7 (psoriasin 1); S100 calcium binding protein A7; S100 calcium-binding protein A7 (psoriasin 1); S100 calcium-binding protein A7; S100A7; S100A7c

Citation for Recombinant Human S100A7 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Activation of the S100A7/RAGE Pathway by IGF-1 Contributes to Angiogenesis in Breast Cancer
    Authors: MG Muoio, M Talia, R Lappano, AH Sims, V Vella, F Cirillo, L Manzella, M Giuliano, M Maggiolini, A Belfiore, EM De Frances
    Cancers, 2021-02-04;13(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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