Recombinant Human SCF Protein, Animal-Free

Name: Recombinant Human SCF Protein, Animal-Free
Brand: R&D Systems
SKU: BT-SCF-AFL
Price: 472 USD

Catalog #: BT-SCF-AFL Datasheet / COA / SDS

GMP Version Available: BT-SCF-GMP

GMP

Intended for preclinical researchers who may transition to GMP SCF for their clinical work

Catalog #	Availability	Size / Price	Qty
BT-SCF-AFL-01M
BT-SCF-AFL-050

Request an Animal-Free Protein Sample. For a limited time, we are offering customers the opportunity to try a sample of any Animal-Free RUO protein at no charge. Request a sample of Animal-Free SCF/c-kit Ligand.

Recombinant Human SCF Protein, Animal-Free, Bioactivity.

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All SCF/c-kit Ligand Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human SCF Protein, Animal-Free Summary

Learn more about Animal-Free Recombinant Proteins

Product Specifications

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 1.00-8.00 ng/mL.

The specific activity of Animal-Free™ Recombinant Human SCF/c‑kit Ligand is >1.0 x 10⁶ units/mg, which is calibrated against the human SCF reference standard (NIBSC code: 91/682).

Source

E. coli-derived human SCF/c-kit Ligand protein
Glu26-Ala189, with a N-terminal Met.
Produced using non-animal reagents in an animal-free laboratory.

Accession #

P21583.1

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

19 kDa

SDS-PAGE

18 kDa, under reducing conditions.

Product Datasheets

BT-SCF-AFL (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT-SCF-AFL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Animal-Free^TM Recombinant Human SCF (Catalog # BT-SCF-AFL) as measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 1.00-8.00 ng/mL.Two independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of Animal-Free SCF.

Bioactivity

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Equivalent bioactivity of GMP (BT-SCF-GMP), Animal-Free (Catalog # BT-SCF-AFL) and RUO (BT-SCF) grades of Recombinant Human SCF as measured in a cell proliferation assay using TF-1 human erythroleukemic cells (orange, green, red, respectively).

SDS-PAGE

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2 μg/lane of Animal-Free™ Recombinant Human SCF/c‑kit Ligand Protein (Catalog # BT-SCF-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18 kDa.

Reconstitution Calculator

Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28‑40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1‑7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N‑linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%‑87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c‑kit to trigger receptor dimerization and signaling (14).

SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15). SCF is a versatile factor in the differentiation of many specific cell types like spermatogonial stem cells (16) and megakaryocyte progenitors (17). Apart from differentiation, SCF also can maintain stemness in cells. This is the case for human bone marrow mesenchymal cells, which require SCF and hepatocyte growth factor for maintenance (18). Hematopoietic stem cells similarly require SCF from surrounding cells in their niche to maintain their stemness and their progenitors (19). SCF has also improved protocols for continuous generation of cells in culture systems, like granulocytes and macrophages (20).

For treatment of graft versus host disease, SCF is used in combination with other cytokines to generate myeloid-derived suppressor cells from human umbilical cord blood (21). SCF is also used to generate T cells for cell-based therapies, drug screening and disease modeling (22). In regenerative studies, SCF is applied in wound healing hydrogel as a means of increasing its adhesion strength and tissue regeneration (23).

References

Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599.
Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201.
Kapur, R. et al. (2002) Blood 100:1287.
Wang, C.H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.
Martin, F.H. et al. (1990) Cell 63:203.
Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
Anderson, D.M. et al. (1991) Cell Growth Differ. 2:373.
Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
Nasimi, M. et al. (2021) Reprod Sci. 28:963.
Krisch, L. et al. (2021) Int. J. Mol. Sci. 22:8224.
Cao, Z. et al. (2020) Stem Cell Res Ther. 11:1.
Comazzetto, S. et al. (2019) Cell Stem Cell. 24:477.
Bernecker, C. et al. (2019) Stem Cells Dev. 28:1540.
Park, M.Y. et al. (2019) Front Immunol. 10:1.
Netsrithong, R. et al. (2020) Stem Cell Res Ther. 11:1.
Zhang, Li. et al. (2021) Journal Mater Chem B. 29:5887.

Long Name

Stem Cell Factor

Entrez Gene IDs

4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)

Alternate Names

c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement