Recombinant Human SMOC-2 Protein, CF Summary
Product Specifications
When 5 x 104 cells/well are added to rhSMOC-2 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED50 for this effect is 0.4‑1.6 μg/mL.
Gln22-Gly446, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5140-SM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: SMOC-2
SMOC-2 (secreted, or SPARC-related, modular calcium-binding protein 2), previously called SMAP2 (smooth muscle-associated protein 2), is a 55 kDa glycoprotein that is a member of the SPARC family of matricellular proteins (1, 2). The human SMOC-2 cDNA encodes 446 amino acids (aa), including a 21 aa signal sequence, a Kazal-like domain (aa 40 - 84), two thyroglobulin type-1 segments (aa 87 - 153 and 213 - 281) and two EF-hand sequences (aa 347 - 382 and 384 - 419) (1, 2). Of three splice variants, one shows a 13 aa substitution for aa 442 - 446, another shows an 11 aa insertion after Thr170, and a third contains both of these variations. Mature human SMOC-2 shares 95%, 95% and 94% aa identity with mouse, rat and canine SMOC-2, respectively. SMOC-2 is widely expressed in the extracellular matrix and appears to have adhesion-related functions (1 - 5). Recombinant bacterially produced human SMOC-1 and SMOC-2 can bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin, while keratinocyte SMOC-2 binds integrins alpha v beta 1 and alpha v beta 6 (3, 6). SMOC-2 promotes cell cycle progression by signaling through the integrin-linked kinase (ILK) to upregulate cyclin-D1 (4). When expressed in the endothelial extracellular matrix, it potentiates growth factor-induced angiogenesis (5). SMOC-2 expression is upregulated during neointima formation, promoting proliferation and migration of vascular smooth muscle (2). In the skin, it promotes keratinocyte attachment and migration (6). Since Kazal and thyroglobulin domains are often found in protease inhibitors, SMOC-2 is also proposed to inhibit proteases in the lung and artery (2, 7).
- Vannahme, C. et al. (2003) Biochem. J. 373:805.
- Nishimoto, S. et al. (2002) Biochim. Biophys. Acta 1576:225.
- Novinec, M. et al. (2008) Protein Expr. Purif. 62:75.
- Liu, P. et al. (2008) Mol. Biol. Cell 19:248.
- Rocnik, E.F. et al. (2006) J. Biol. Chem. 281:22855.
- Maier, S. et al. (2008) Exp. Cell Res. 314:2477.
- Wilk, J.B. et al. (2007) Am. J. Respir. Crit. Care Med. 175:554.
Citations for Recombinant Human SMOC-2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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SMOC2 promotes an epithelial-mesenchymal transition and a pro-metastatic phenotype in epithelial cells of renal cell carcinoma origin
Authors: D Feng, P Gao, N Henley, M Dubuissez, N Chen, LP Laurin, V Royal, V Pichette, C Gerarduzzi
Cell Death & Disease, 2022-07-22;13(7):639.
Species: Canine, Human
Sample Types: Whole Cells
Applications: Bioassay -
SMOC2, an intestinal stem cell marker, is an independent prognostic marker associated with better survival in colorectal cancers
Authors: BG Jang, HS Kim, JM Bae, WH Kim, HU Kim, GH Kang
Sci Rep, 2020-09-03;10(1):14591.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
SMOC Binds to Pro-EGF, but Does Not Induce Erk Phosphorylation via the EGFR
Authors: JT Thomas, L Chhuy-Hy, KR Andrykovic, M Moos
PLoS ONE, 2016-04-21;11(4):e0154294.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
SMOC1 is a tenascin-C interacting protein over-expressed in brain tumors.
Authors: Brellier F, Ruggiero S, Zwolanek D, Martina E, Hess D, Brown-Luedi M, Hartmann U, Koch M, Merlo A, Lino M, Chiquet-Ehrismann R
Matrix Biol., 2011-02-21;30(3):225-33.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
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