Recombinant Human Stabilin-2 (aa 2198-2295) Protein Summary
Product Specifications
| MNHKVHM | Human Stabilin (Gly2198 - Val2295) Accession # Q8WWQ8 |
6-His tag |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3645-S2
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
3645-S2/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Stabilin-2
Stabilin-2, also known as HARE (hyaluronan receptor for endocytosis) and FEEL-2 (fasciclin, EGF-like, laminin type EGF-like, and link domain containing scavenger receptor 2), is a type I transmembrane multi-domain protein that is the closest homolog of stabilin-1 (1, 2). It is a scavenger receptor that is expressed on sinusoidal endothelial cells of liver, spleen, and lymph node (1, 2). Its 2439 amino acid (aa) extracellular domain contains seven fasciclin domains, multiple EGF-like and laminin type EGF-like domains, and a link domain related to molecules of the TSG-6 superfamily (3). The 72 aa cytoplasmic tail of Stabilin-2 contains a motif that allows the AP2 classical cargo adaptor to direct cargo into clathrin-coated pits (4). As a recycling receptor, stabilin-2 cycles from the membrane to clathrin-coated pits each 10 - 15 minutes, with about two thirds of the protein found within the endocytic system at any one time (4). The link domain binds and mediates the systemic clearance of hyaluronan (HA) (2 - 6). Within the link domain (aa 2198 - 2295), human stabilin-2 shares 86% and 89% aa identity with mouse and rat stabilin-2, respectively. Total human HA is about 15 grams, of which about 5 grams are cleared each day (5). Stabilin-2 also mediates the endocytosis of chondroitin and chondroitin sulfate, advanced glycosylation end-product (AGE), collagen N-terminal propeptides and acetylated LDL (4 - 7). Human stabilin-2 mRNA encodes a 2251 aa, 315 kDa protein that produces an isoform of 190 kDa through proteolytic cleavage (2, 6). The 190 kDa form lacks some N-terminal fasciclin and EGF-like domains, but shows similar activities when compared with the 315 kDa form (2, 6).
- Politz, O. et al. (2002) Biochem. J. 362:155.
- Zhou, B. et al. (2003) Glycobiology 13:339.
- Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
- Hansen, B. et al. (2005) Exp. Cell Res. 303:160.
- Harris, E.N. et al. (2004) J. Biol. Chem. 279:36201.
- Harris, E.N. et al. (2007) J. Biol. Chem. 282:2785.
- Tamura, Y. et al. (2003) J. Biol. Chem. 278:12613.
Citation for Recombinant Human Stabilin-2 (aa 2198-2295) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Highly sensitive quantification of bacterial chondroitin in a culture based on ELISA techniques
Authors: K Suzuki, H Kaseyama-T, S Ito
Journal of microbiological methods, 2022-09-16;0(0):106579.
Species: Bacteria - Escherichia coli
Sample Types: Protein
Applications: Surface Plasmon Resonance (SPR
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