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Recombinant Human Testican 2/SPOCK2 Protein, CF

Catalog #: 2328-PI Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2328-PI-050
R&D Systems Recombinant Proteins and Enzymes
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Testican 2/SPOCK2 Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived human Testican 2/SPOCK2 protein
Glu23-Trp424, with a C-terminal 6-His tag
Accession #
Q92563
N-terminal Sequence
Analysis
Glu23
Predicted Molecular Mass
45.4 kDa
SDS-PAGE
62-65 kDa, reducing conditions

Product Datasheets

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2328-PI

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2328-PI

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Testican 2/SPOCK2

Testican 2 encoded by the SPOCK2 gene is a proteoglycan highly expressed in brain (1, 2). The human and mouse cDNAs predict 94% identity between the deduced amino acid sequences from the two species, indicating a conserved function (3, 4). Testican 2 contains a Ca2+-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. However, these domains may not be involved directly in the inhibition of targeted proteases. For example, Testican 2 is not able to inhibit MMP-14 (MT1-MMP, a metalloprotease) activation of MMP-2 (5). The purified rhTestican 2 is not capable of inhibiting rhMMP-14 and rhCathepsin L (R&D Systems, Catalog # 918-MP and 952-CY) in assays using the fluorogenic peptide substrates (R&D Systems, Catalog # ES001 and ES008). Nevertheless, over expression of Testican 2 is able to abrogate inhibition of MMP-14 by other testican family members and to inhibit neurite outgrowth (6, 7). 

References
  1. Nagase, T. et al. (1996) DNA Res. 3:321.
  2. Vannahme, C. et al. (1999) J. Neurochem. 73:12.
  3. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
  4. Okazaki, Y. et al. (2002) Nature 420:563.
  5. Nakada, M. et al. (2001) Cancer Res. 61:8896.
  6. Nakada, M. et al. (2003) Cancer Res. 63:3364.
  7. Hartmann, U. and P. Maurer (2001) Matrix Biol. 20:23.
Entrez Gene IDs
9806 (Human)
Alternate Names
FLJ97039; KIAA0275; KIAA0275CWCV, and Kazal-like domains proteoglycan 2; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2; SPOCK2; Testican 2

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