Can Recombinant Human TGF-β RII (Catalog # 241-R2) be used as a capture reagent in an ELISA-like assay?

Name: Recombinant Human TGF-beta RII Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 341-BR
Price: 564 USD

Yes, it can, but Recombinant Human TGF-β RII Fc Chimera (Catalog # 341-BR) binds to the plate better and has a higher affinity for the ligand. Therefore, Catalog # 341-BR is recommended to use as a capture reagent in ELISA applications.

Recombinant Human TGF-beta RII Fc Chimera Protein, CF

Catalog #: 341-BR
9 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
341-BR-050

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Citations (9)

FAQs

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TGF-beta RII Fc Chimera Protein, CF Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human TGF‑ beta 1 (Catalog # 240-B/CF) is immobilized at 0.5 µg/mL, the concentration of Recombinant Human TGF‑ beta RII Fc Chimera that produces 50% of the optimal binding response is found to be approximately 6-30 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human TGF-beta RII protein

Human TGF-beta RII (Thr23-Asp159) Accession # P37173	MD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

P37173

N-terminal Sequence
Analysis

Thr23

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41.7 kDa (monomer)

SDS-PAGE

59 kDa-66 kDa, reducing conditions

Product Datasheets

341-BR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

341-BR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute with PBS to prepare a stock solution of 100 μg/mL.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.

References

Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.

Long Name

Transforming Growth Factor beta Receptor II

Entrez Gene IDs

7048 (Human); 21813 (Mouse)

Alternate Names

AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II

Related Research Areas

Citations for Recombinant Human TGF-beta RII Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Circulating Picomolar Levels of CCL2 Downregulate Ongoing Chronic Experimental Autoimmune Encephalomyelitis by Induction of Regulatory Mechanisms
Authors: N Kaushansky, E Bakos, S Becker-Her, I Shachar, A Ben-Nun
J. Immunol., 2019-09-04;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Bifunctional immune checkpoint-targeted antibody-ligand traps that simultaneously disable TGF? enhance the efficacy of cancer immunotherapy
Authors: R Ravi, KA Noonan, V Pham, R Bedi, A Zhavoronko, IV Ozerov, E Makarev, A V Artemov, PT Wysocki, R Mehra, S Nimmagadda, L Marchionni, D Sidransky, IM Borrello, E Izumchenko, A Bedi
Nat Commun, 2018-02-21;9(1):741.
Applications: Bioassay
TGF-? contamination of purified recombinant GDF15
Authors: OE Olsen, A Skjærvik, BF Størdal, A Sundan, T Holien
PLoS ONE, 2017-11-21;12(11):e0187349.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Sulfated Hyaluronan Derivatives Modulate TGF-?1:Receptor Complex Formation: Possible Consequences for TGF-?1 Signaling
Authors: L Koehler, S Samsonov, S Rother, S Vogel, S Köhling, S Moeller, M Schnabelra, J Rademann, U Hempel, MT Pisabarro, D Scharnwebe, V Hintze
Sci Rep, 2017-04-26;7(1):1210.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Role of a Novel Human Leukocyte Antigen-DQA1*01:02;DRB1*15:01 Mixed Isotype Heterodimer in the Pathogenesis of "Humanized" Multiple Sclerosis-like Disease.
Authors: Kaushansky N, Eisenstein M, Boura-Halfon S, Hansen B, Nielsen C, Milo R, Zeilig G, Lassmann H, Altmann D, Ben-Nun A
J Biol Chem, 2015-04-24;290(24):15260-78.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA (Capture)
Induction and activation of latent transforming growth factor-beta1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1).
Authors: Ballesteros A, Mentink-Kane M, Warren J, Kaplan G, Dveksler G
J Biol Chem, 2014-12-29;290(7):4422-31.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Bioassay
Anterior gradient 2 downregulation in a subset of pancreatic ductal adenocarcinoma is a prognostic factor indicative of epithelial-mesenchymal transition.
Authors: Mizuuchi Y, Aishima S, Ohuchida K, Shindo K, Fujino M, Hattori M, Miyazaki T, Mizumoto K, Tanaka M, Oda Y
Lab Invest, 2014-11-24;95(2):193-206.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Downregulation of Dickkopf-3 disrupts prostate acinar morphogenesis through TGF-beta/Smad signalling.
Authors: Romero D, Kawano Y, Bengoa N, Walker M, Maltry N, Niehrs C, Waxman J, Kypta R
J Cell Sci, 2013-02-26;126(0):1858-67.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Regulating human Th17 cells via differential expression of IL-1 receptor.
Authors: Lee WW, Kang SW, Choi J
Blood, 2009-11-12;115(3):530-40.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

FAQs

Can Recombinant Human TGF-β RII (Catalog # 241-R2) be used as a capture reagent in an ELISA-like assay?
- Yes, it can, but Recombinant Human TGF-β RII Fc Chimera (Catalog # 341-BR) binds to the plate better and has a higher affinity for the ligand. Therefore, Catalog # 341-BR is recommended to use as a capture reagent in ELISA applications.

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