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Recombinant Human uPAR Fc Chimera Protein, CF

Catalog #: 10378-UK Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10378-UK-100
Recombinant Human uPAR Fc Chimera Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human uPAR Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When Recombinant Human uPAR Fc Chimera (Catalog # 10378-UK) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human u-Plasminogen Activator (uPA)/Urokinase (Catalog # 1310-SE) binds with an ED50 of 2-16 ng/mL.

Source
Chinese Hamster Ovary cell line, CHO-derived human uPAR protein
Human uPAR
(Leu23-Arg303)
Accession # Q03405.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q03405.1
N-terminal Sequence
Analysis
Leu23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
58 kDa
SDS-PAGE
74-85 kDa, under reducing conditions

Product Datasheets

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10378-UK

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10378-UK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human uPAR Fc Chimera Protein Binding Activity View Larger

When Recombinant Human uPAR Fc Chimera (Catalog # 10378-UK) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) binds with an ED50 of 2-16 ng/mL.

SDS-PAGE Recombinant Human uPAR Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human uPAR Fc Chimera (Catalog # 10378-UK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-85 kDa and 150- 170 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: uPAR

uPAR (urokinase-type plasminogen activator receptor, also known as CD87), is a cell surface receptor that binds urokinase-type plasminogen activator (uPA) which cleaves the zymogen plasminogen to form the active enzyme plasmin. uPAR expression is activated during inflammation, immune responses, and conditions of tissue remodeling and wound healing (1). Human uPAR is synthesized as a 335 amino acid (aa) residue precursor protein with a 22 aa signal peptide. Removal of the C-terminal propeptide generates a fully processed uPAR containing 283 aa with five potential N-linked glycosylation sites and a C-terminal GPI-anchor site (2). Variants due to alternative splicing of uPAR also exist (3). Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific (4). The uPA/uPAR interaction initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR is considered as a biomarker in many inflammatory diseases including cancer, cardiovascular diseases, chronic kidney diseases and diabetes (5).

References
  1. Smith, H.W. and C.J. Marshall (2010) Nat. Rev. Mol. Cell Biol. 11:23.
  2. Ploug, M. et al. (1991) J. Biol. Chem. 266:1926.
  3. Dear, A.E. and R.L. Medcalf (1998) Eur. J. Biochemistry 252:185.
  4. Ellis, V. et al. (1992) Ann. N.Y. Acad. Sci. 667:13.
  5. Desmedt, S. et al. (2017) Crit. Rev. Clin. Lab. Sci. 54:117.
Long Name
Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs
5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)
Alternate Names
CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3

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