Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF
Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
Human VE-Cadherin (Asp48-Gln593) Accession # P33151 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
938-VC
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: VE-Cadherin
VE-Cadherin, also known as Cadherin-5 and CD144, is a member of the atypical/type II subgroup of Cadherin homophilic adhesion proteins (1). Mature human VE-Cadherin is a 125-135 kDa transmembrane glycoprotein that consistis of a 552 amino acid (aa) extracellular domain (ECD) with five Ca++-binding Cadherin domains, a 21 aa transmembrane segment, and a 164 aa cytoplasmic domain (2, 3). Within the ECD, human VE-Cadherin shares approximately 75% aa sequence identity with mouse and rat VE-Cadherin. A 90 kDa portion of the VE-Cadherin ECD can be shed in a metalloproteinase-dependent mechanism and may function as a VE-Cadherin antagonist (4, 5). VE-Cadherin is expressed on the surface of vascular endothelial cells (EC) from early in embryogenesis through adulthood as well as in the placenta, on hematopoietic cell progenitors, and on a subpopulation of hematopoietic stem cells (6-8). It is a major protein component of adherens junctions between cells of the endothelium (9). VE-Cadherin supports the extension and stabilization of existing vascular sprouts (10). VE-Cadherin also regulates or is regulated by VEGF R2, type I and type II TGF-beta receptors, and other endothelial junction proteins such as JAM-C, Claudin-5, and N-Cadherin (11-15). Elevated levels of soluble VE-Cadherin are found in the serum of myocardial infarction, angina pectoris, rheumatoid arthritis, and colorectal cancer patients (16-18).
- Giannotta, M. et al. (2013) Dev. Cell 26:441.
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- Li, H. et al. (2010) Cancer Gene Ther. 17:700.
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- Perryn, E.D. et al. (2008) Dev. Biol. 313:545.
- Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
- Orlova, V.V. et al. (2006) J. Exp. Med. 203:2703.
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- Soeki, T. et al. (2004) Circ. J. 68:1.
- Sulkowska, M. et al. (2006) Tumori 92:67.
- Sidibe, A. et al. (2012) Arthritis Rheum. 64:77.
Citations for Recombinant Human VE-Cadherin Fc Chimera His-tag Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Neuropilin 1 and its inhibitory ligand mini-tryptophanyl-tRNA synthetase inversely regulate VE-cadherin turnover and vascular permeability
Authors: N Gioelli, LJ Neilson, N Wei, G Villari, W Chen, B Kuhle, M Ehling, F Maione, S Willox, S Brundu, D Avanzato, G Koulouras, M Mazzone, E Giraudo, XL Yang, D Valdembri, S Zanivan, G Serini
Nature Communications, 2022-07-20;13(1):4188.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Mutant B-Raf(V600E) Promotes Melanoma Paracellular Transmigration by Inducing Thrombin-mediated Endothelial Junction Breakdown.
Authors: Zhang P, Feng S, Liu G, Wang H, Zhu H, Ren Q, Bai H, Fu C, Dong C
J Biol Chem, 2015-10-26;291(5):2087-106.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cadherin 6 has a functional role in platelet aggregation and thrombus formation.
Authors: Dunne E, Spring CM, Reheman A, Jin W, Berndt MC, Newman DK, Newman PJ, Ni H, Kenny D
Arterioscler. Thromb. Vasc. Biol., 2012-04-26;32(7):1724-31.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Lentiviral rescue of vascular endothelial growth factor receptor-2 expression in flk1-/- embryonic stem cells shows early priming of endothelial precursors.
Authors: Li X, Edholm D, Lanner F, Breier G, Farnebo F, Dimberg A, Claesson-Welsh L
Stem Cells, 2007-08-16;25(12):2987-95.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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