Recombinant Influenza A Virus H3N2 Hemagglutinin Protein, CF
Recombinant Influenza A Virus H3N2 Hemagglutinin Protein, CF Summary
Product Specifications
Gln17-Trp530, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10944-HA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human Galectin-1 (1152-GA) immobilized at 10.00 μg/mL, 100 μL/well, the concentration of Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag (Catalog # 10944-HA) that produces 50% of the optimal binding response is 0.35 - 3.50 μg/mL.
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2 μg/lane of Recombinant Influenza A Virus H3N2 Hemagglutinin His-tag Protein (Catalog # 10944-HA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-90 kDa.
Reconstitution Calculator
Background: Hemagglutinin
Influenza A (H3N2) has predominated recent influenza seasons, which caused several hospitalizations in many countries (1). H3N2 viruses have been in circulation since the onset of the 1968 pandemic (2). This strain was a reassortant, composed of the six gene segments from H2N2 viruses, but with the hemagglutinin and polymerase basic protein 1 segments derived from an avian source (3, 4). The hemagglutinin protein of the Influenza A virus (strain A/Hong Kong/1/1968 H3N2) is composed of 550 residues, which includes the Hemagglutinin chains HA1 and HA2 (5). Hemagglutinin (HA) and Neuraminidase (NA) and are the two predominant membrane glycoproteins found on the surface of influenza virus. HA is a lectin that binds sialic acid on host cell membrane. NA is a sialic acid hydrolase that specifically clips off terminally located sialic acid on host cell surface. The two proteins are essential for the infectious cycle of the influenza virus. Galectin-1 can bind to the envelope glycoproteins of influenza virus and inhibit viral infectivity, which may be attributed to the multivalent binding and cross-linking activities of galectin-1. Furthermore, galectin-1 may also be explored for targeting other viruses with glycoproteins on their surface (6).
- Wan, H. et al. (2019) Nat. Microbiol. 4:2216.
- Krause, J.C. et al. (2012) J. Virol. 86:6334.
- Kawaoka, Y. et al. (1989) J. Virol. 63:4603.
- Alymova, I.V. et al. (2011) J. Virol. 85:12324.
- Brown, E.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6883.
- Yang, M.L. et al. (2011) J. Virol. 85:10010.
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