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Recombinant Mouse Activin B Protein

Catalog #: 8260-AB
5 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
8260-AB-010/CF

With Carrier

Catalog # Availability Size / Price Qty
8260-AB-010
Recombinant Mouse Activin B Protein Bioactivity
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Product Details
Citations (5)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Activin B Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2-1.2 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived mouse Activin B protein
Gly297-Ala411
Accession #
Q04999
N-terminal Sequence
Analysis
Gly297
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
13 kDa (monomer)
SDS-PAGE
12 kDa, reducing conditions

Product Datasheets

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8260-AB (with carrier)

Product Datasheet
COA
SDS

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8260-AB/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8260-AB

Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8260-AB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Activin B Protein Bioactivity View Larger

Recombinant Mouse Activin B (Catalog # 8260-AB) induces hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.2-1.2 ng/mL.

SDS-PAGE Recombinant Mouse Activin B Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse Activin B was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 12 and 21 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Activin B

Activin and Inhibin, members of the TGF-beta superfamily of cytokines, are involved in a range of biological processes including neural development, stem cell differentiation, reproductive physiology, inflammation, tissue morphogenesis and bone remodeling (1-5). Activins function as either a homodimer or heterodimer of non-glycosylated beta subunit proteins ( beta A, beta B, beta C, and beta E in mammals). Inhibins are heterodimers consisting of a unique alpha  subunit and any one beta  subunit. The alpha - and beta -subunits are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release a carboxy-terminal monomeric subunit that becomes bioactive upon disulfide linkage with another Activin monomer (6, 7). The bioactive Activin B dimeric protein consists of two beta B subunits. The 13 kDa mature mouse Activin beta B subunit shares 99% and 98% amino acid sequence identity with rat and human Activin beta B, respectively. Activin B exerts its biological function upon binding to the type 2 serine/threonine kinase Activin receptor (Act RIIA). This receptor-ligand complex noncovalently associates and transphosphorylates the type 1 Activin receptor (ActRI) to initiate intracellular SMAD activation and the subsequent regulation of Activin-responsive gene transcription (8). BAMBI, Betaglycan, and Cripto, regulate the bioactivity of Activin B by restricting its ability to induce receptor complex assembly. Alternatively, bioavailability of Activin B is modulated through sequestration into inactive complexes with alpha 2-Macroglobulin, Follstatin, and FLRG (9-12).

References
  1. Woodruff, T.K. (1998) Biochemical Pharmacology 55:953.
  2. Paulusma, C.C. et al. (1994) J. Immunol. Methods 169:143.
  3. Vassalli, A. et al. (1994) Genes Dev. 8:414.
  4. Shat-Tal, Y. and D. Zipori (2002) Stem Cells 20:493.
  5. Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114.
  6. Gray, A.M. and A.J. Mason (1990) Science 247:1328.
  7. Mason, A.J. et al. (1996) Mol. Endocrinol. 10:1055.
  8. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  9. Hedger, M.P. and D.M. de Kretser (2013) Cytokine Growth Factor Rev. 24: 285.
  10. Gray, P.C. and W. Vale (2012) FEBS Letters 586:1836.
  11. Kelber, J.A. et al. (2008) J. Biol. Chem. 283: 4490.
  12. Xia, Y. and A.L. Schneyer (2009) Journal of Endocrinology 202:1.
Long Name
Inhibin Subunit Beta B
Entrez Gene IDs
3625 (Human); 3625 (Human); 16324 (Mouse); 16324 (Mouse); 25196 (Rat); 25196 (Rat)
Alternate Names
activin AB beta polypeptide; Activin Beta B; Activin beta-B chain; INHBB; inhibin beta B chain; Inhibin Beta B; Inhibin Subunit Beta B; inhibin, beta B (activin AB beta polypeptide); inhibin, beta B; Inhibin, beta-2

Citations for Recombinant Mouse Activin B Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Factors affecting the induction of uncoupling protein 1 in C2C12 myogenic cells
    Authors: T Yamamoto, Z Diao, M Murakami, F Shimokawa, T Matsui, M Funaba
    Cytokine, 2022-06-20;157(0):155936.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. mDia1 and Cdc42 regulate Activin B-induced migration of bone marrow-derived mesenchymal stromal cells
    Authors: X Wang, P Tang, F Guo, M Zhang, Y Yan, M Huang, Y Chen, L Zhang, L Zhang
    Stem Cells, 2018-11-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Activin Receptor Type IIA (ACVR2A) Functions Directly in Osteoblasts as a Negative Regulator of Bone Mass
    Authors: BC Goh, V Singhal, AJ Herrera, RE Tomlinson, S Kim, MC Faugere, EL Germain-Le, TL Clemens, SJ Lee, DJ DiGirolamo
    J. Biol. Chem., 2017-06-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Activin B regulates adipose-derived mesenchymal stem cells to promote skin wound healing via activation of the MAPK signaling pathway
    Authors: L Zhang, P Xu, X Wang, M Zhang, Y Yan, Y Chen, L Zhang, L Zhang
    Int. J. Biochem. Cell Biol., 2017-04-08;87(0):69-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Adipocyte ALK7 links nutrient overload to catecholamine resistance in obesity.
    Authors: Guo T, Marmol P, Moliner A, Bjornholm M, Zhang C, Shokat K, Ibanez C
    Elife, 2014-08-25;3(0):e03245.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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