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Recombinant Mouse Angiopoietin-3 Protein

Catalog #: 738-AN
1 Citation Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
738-AN-025/CF

With Carrier

Catalog # Availability Size / Price Qty
738-AN-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (1)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Angiopoietin-3 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rmTie-2/Fc Chimera at 4 µg/mL (100 µL/well) can bind rmAngiopoietin-3 with a linear range of 5-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Angiopoietin-3 protein
Mouse Angiopoietin-3
(Gln22 – Ala509)
Accession # Q9WVH6		 IEGR GGGSGGGSGGGS 10-His tag
N-terminus C-terminus
Accession #
Q9WVH6
N-terminal Sequence
Analysis
No results obtained: Gln22 predicted
Structure / Form
Oligomer
Predicted Molecular Mass
58 kDa
SDS-PAGE
85-90 kDa, reducing conditions

Product Datasheets

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738-AN (with carrier)

Product Datasheet
COA
SDS

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738-AN/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

738-AN

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

738-AN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-3

Mouse angiopoietin-3 (ANG-3) (1), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Mouse ANG-3 cDNA encodes a 509 amino acid (aa) precursor protein with a 21 aa signal peptide. It shares 47%, 46% and 54% aa sequence identity with mouse ANG-1, mouse ANG-2 and human ANG-4, respectively. Although the sequence homology is much higher between the human and mouse counterparts for ANG-1 (97%) and ANG-2 (85%), mouse ANG-3 is believed to be an ortholog of human ANG-4 based on chromosomal localization studies (1, 2). Human ANG-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse ANG-3 is expressed in multiple mouse tissues. Human ANG-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse ANG-3 has been reported to be a Tie-2 antagonist. It is likely that mouse ANG-3, like ANG-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

References
  1. Valenzuela, D.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1904.
  2. Nishimura, M. et al. (1999) FEBS Lett. 448:254.
  3. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
  4. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.
Entrez Gene IDs
11602 (Mouse)
Alternate Names
ANG3; ANG4; Angiopoietin-3; ANGPT3

Citation for Recombinant Mouse Angiopoietin-3 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina
    Authors: H Elamaa, M Kihlström, E Kapiainen, M Kaakinen, I Miinalaine, S Ragauskas, M Cerrada-Gi, S Mering, M Nätynki, L Eklund
    Elife, 2018-11-16;7(0):.
    Species: Human
    Sample Types: Recombinant Protein, Whole Cells
    Applications: Bioassay, ELISA (Capture), Surface Plasmon Resonance

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