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Recombinant Mouse CCL20/MIP-3 alpha Protein

Catalog #: 760-M3
14 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
760-M3-025/CF

With Carrier

Catalog # Availability Size / Price Qty
760-M3-025
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Citations (14)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CCL20/MIP-3 alpha Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR6. The ED 50 for the chemotactic effect is 2-6 ng/mL.
Source
E. coli-derived mouse CCL20/MIP-3 alpha protein Ala27-Met96
Accession #
Q642U4
N-terminal Sequence
Analysis
Ala27
Predicted Molecular Mass
7.9 kDa

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760-M3 (with carrier)

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760-M3/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

760-M3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.

760-M3/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL20/MIP-3 alpha

MIP-3 alpha, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20 - 28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71 and 63% amino acid sequence homology with rat and human MIP-3 alpha, respectively. MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34 + cord blood precursors.

References
  1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
  2. Hromas, R. et al. (1997) Blood 89:3315.
  3. Greaves, D. R. et al. (1997) J. Exp. Med. 186: 857.
  4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.
Entrez Gene IDs
6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names
beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

Citations for Recombinant Mouse CCL20/MIP-3 alpha Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Pertussis Toxin Inhibits Encephalitogenic T-Cell Infiltration and Promotes a B-Cell-Driven Disease during Th17-EAE
    Authors: Z Maria, E Turner, A Agasing, G Kumar, RC Axtell
    International Journal of Molecular Sciences, 2021-03-13;22(6):.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Bioassay
  2. A chemokine-fusion vaccine targeting immature dendritic cells elicits elevated antibody responses to malaria sporozoites in infant macaques
    Authors: K Luo, JT Gordy, F Zavala, RB Markham
    Scientific Reports, 2021-01-13;11(1):1220.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Capture
  3. Effective chemoimmunotherapy by co-delivery of doxorubicin and immune adjuvants in biodegradable nanoparticles
    Authors: CG Da Silva, MGM Camps, TMWY Li, L Zerrillo, CW Löwik, F Ossendorp, LJ Cruz
    Theranostics, 2019-08-21;9(22):6485-6500.
    Species: Mouse
    Sample Types: In Vivo, Nanoparticle
    Applications: Bioassay, In Vivo
  4. Protection from EAE in DOCK8 mutant mice occurs despite increased Th17 cell frequencies in the periphery
    Authors: AS Wilson, HD Law, CB Knobbe-Tho, CJ Kearney, J Oliaro, C Binsfeld, G Burgio, L Starrs, D Brenner, KL Randall, A Brüstle
    Eur. J. Immunol., 2019-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Leukocyte-endothelial cell interaction is enhanced in podocalyxin-deficient mice
    Authors: G Porras, MS Ayuso, C González-M
    Int. J. Biochem. Cell Biol., 2018-03-28;99(0):72-79.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
    Authors: Valentina Cecchinato
    J. Immunol, 2016-11-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. P2X7 receptor-dependent tuning of gut epithelial responses to infection
    Authors: SW Huang, C Walker, J Pennock, K Else, W Muller, MJ Daniels, C Pellegrini, D Brough, G Lopez-Cast, SM Cruickshan
    Immunol. Cell Biol, 2016-08-25;95(2):178-188.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin
    Authors: SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes
    J. Immunol, 2016-02-05;196(6):2514-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFkappaB and ERK.
    Authors: Badr G, Sayed A, Abdel-Maksoud M, Mohamed A, El-Amir A, Abdel-Ghaffar F, Al-Quraishy S, Mahmoud M
    PLoS ONE, 2015-04-24;10(4):e0125340.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis.
    Authors: Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, Hacker G, Schmetzer H, Hacker H, Hammerschmidt S, van der Ende A, Pfister H, van de Beek D, Koedel U
    PLoS ONE, 2014-04-03;9(4):e93057.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
    Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
    Eur. J. Immunol., 2012-05-01;42(5):1250-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control.
    Authors: Cha HR, Ko HJ, Kim ED
    J. Immunol., 2011-08-10;187(6):3044-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. A novel role for constitutively expressed epithelial-derived chemokines as antibacterial peptides in the intestinal mucosa.
    Authors: Kotarsky K, Sitnik KM, Stenstad H, Kotarsky H, Schmidtchen A, Koslowski M, Wehkamp J, Agace WW
    Mucosal Immunol, 2009-10-07;3(1):40-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Astrocytes are the major intracerebral source of macrophage inflammatory protein-3alpha/CCL20 in relapsing experimental autoimmune encephalomyelitis and in vitro.
    Authors: Ambrosini E, Columba-Cabezas S, Serafini B, Muscella A, Aloisi F
    Glia, 2003-02-01;41(3):290-300.
    Species: Mouse
    Sample Types: N/A, Whole Cells
    Applications: Bioassay, Western Blot

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