Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119) Protein
Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119) Protein Summary
Product Specifications
In the same assay, the ED50 of the aa 27-119 mouse Eotaxin-2 variant (R&D Systems, Catalog # 1017-MP) is 1-5 ng/mL. The aa 24-119 mouse Eotaxin-2 variant is about 150-fold less active than the aa 27-119 mouse Eotaxin-2 variant.
Ile24-Val119
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
528-MP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
528-MP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CCL24/Eotaxin-2/MPIF-2
Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin. Two amino-terminally-truncated isoforms of mouse Eotaxin-2 have been produced at R&D Systems. The Val 27 - Val 119 mouse Eotaxin-2 isoform has been shown to be 150-fold more potent than the Ile 24 - Val 119 isoform as a chemoattractant for mouse BaR/3 cells transfected with mouse CCR3 (1 - 3).
- Zimmermann, N. et al. (2000) J. Immunol. 165:5839.
- Watanabe, K. et al. (2002) J. Immunol. 168:1911.
- Grzegorzewski, K.J. et al. (2001) Cytokine 13:209.
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