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Recombinant Mouse CD163 Protein, CF

Catalog #: 7435-CD
1 Citation Datasheet / COA / SDS
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7435-CD-050
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD163 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD163 is immobilized at 5 μg/mL (100 μL/well), the concentration of mouse Haptoglobin/Hemoglobin (Hp/Hb) that produces 50% optimal binding response is approximately 0.2-1 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD163 protein
Val39-Thr1045, with a C-terminal 10-His tag
Accession #
AAK16065
N-terminal Sequence
Analysis
Val39
Structure / Form
Monomer
Predicted Molecular Mass
110 kDa
SDS-PAGE
130-160 kDa, reducing conditions

Product Datasheets

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7435-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7435-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD163

CD163, also known as M130 and  p155, is a 130-160 kDa type I transmembrane protein belonging to group B of the cysteine-rich scavenger receptor family (1). Mature mouse CD163 consists of a 1007 amino acid (aa) extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (2). Alternative splicing of mouse CD163 generates an isoform with a substitution in the cytoplasmic region. Within the ECD, mouse CD163 shares 75% and 90% aa sequence identity with human and rat CD163, respectively. CD163 is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10 (3-5). A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage (6, 7) in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6 (8-10). CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines (11). It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin (12, 13). It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion (14). CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis (15, 16).

References
  1. Kowal, K. et al. (2011) Folia Histochem. Cytobiol. 49:365.
  2. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
  3. Hogger, P. et al. (1998) J. Immunol. 161:1883.
  4. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
  5. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
  6. Etzerodt, A. et al. (2010) J. Leukoc. Biol. 88:1201.
  7. Moreno, J.A. et al. (2012) Eur. Heart J. 33:252.
  8. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
  9. Timmermann, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
  10. Sulahian, T.H. et al. (2004) J. Leukoc. Biol. 76:271.
  11. Fabriek, B.O. et al. (2009) Blood 113:887.
  12. Kristiansen, M. et al. (2001) Nature 409:198.
  13. Schaer, D.J. et al. (2006) Blood 107:373.
  14. Fabriek, B.O. et al. (2007) Blood 109:5223.
  15. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
  16. Moreno, J.A. et al. (2009) Atherosclerosis 207:103.
Entrez Gene IDs
9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names
CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163

Citation for Recombinant Mouse CD163 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Precision Targeting of Tumor Macrophages with a CD206 Binding Peptide
    Authors: P Scodeller, L Simón-Grac, S Kopanchuk, A Tobi, K Kilk, P Säälik, K Kurm, ML Squadrito, VR Kotamraju, A Rinken, M De Palma, E Ruoslahti, T Teesalu
    Sci Rep, 2017-11-07;7(1):14655.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay

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