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Recombinant Mouse DLL1 Fc Chimera Protein, CF

Catalog #: 5026-DL
12 Citations 2 Reviews Datasheet / COA / SDS
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5026-DL-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (12)
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Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse DLL1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.25-1 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse DLL1 protein
Mouse DLL1
(Ser22-Gln516)
Accession # Q61483		 LIEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
Q61483
N-terminal Sequence
Analysis
Ser22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
81.4 kDa (monomer)
SDS-PAGE
94 kDa, reducing conditions

Product Datasheets

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5026-DL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5026-DL

Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and EDTA.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature mouse DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, mouse DLL1 shares 91% and 95% aa sequence identity with human and rat DLL1, respectively. It shares 26%, 35%, and 51% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment, released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (6-12). The up-regulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (13). DLL1 is also over-expressed in cervical carcinoma and glioma and contributes to tumor progression (14, 15).

References
  1. Bettenhausen, B. et al. (1995) Development 121:2407.
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
  3. Karanu, F.N. et al. (2001) Blood 97:1960.
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
  6. Takahashi, Y. et al. (2003) Development 130:4259.
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Shimojo, H. et al. (2008) Neuron 58:52.
  12. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. 104:537.
  13. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  14. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
  15. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.
Long Name
Delta-like 1
Entrez Gene IDs
28514 (Human); 13388 (Mouse); 84010 (Rat)
Alternate Names
delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1

Citations for Recombinant Mouse DLL1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells
    Authors: I Kim, A Ghosh, N Bundschuh, L Hinte, E Petrosyan, F von Meyenn, O Bar-Nur
    Science Advances, 2022-04-06;8(14):eabj4928.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2
    Authors: S Kakuda, RK Lopilato, A Ito, RS Haltiwange
    J. Biol. Chem., 2020-08-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Notch1 signaling in NOTCH1-mutated mantle cell lymphoma depends on Delta-Like ligand 4 and is a potential target for specific antibody therapy
    Authors: E Silkensted, F Arenas, B Colom-Sanm, S Xargay-Tor, M Higashi, A Giró, V Rodriguez, P Fuentes, WE Aulitzky, H van der Ku, S Beà, ML Toribio, E Campo, M López-Guer, D Colomer
    J. Exp. Clin. Cancer Res., 2019-11-01;38(1):446.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling
    Authors: KB Kaylan, IC Berg, MJ Biehl, A Brougham-C, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill
    Elife, 2018-12-27;7(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Impaired Notch Signaling Leads to a Decrease in p53 Activity and Mitotic Catastrophe in Aged Muscle Stem Cells
    Authors: L Liu, GW Charville, TH Cheung, B Yoo, PJ Santos, M Schroeder, TA Rando
    Cell Stem Cell, 2018-09-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Two novel protein O-glucosyltransferases that modify sites distinct from POGLUT1 and affect Notch trafficking and signaling
    Authors: H Takeuchi, M Schneider, DB Williamson, A Ito, M Takeuchi, PA Handford, RS Haltiwange
    Proc. Natl. Acad. Sci. U.S.A., 2018-08-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development
    Authors: T Kostrzewsk, AJ Borg, Y Meng, I Filipovic, V Male, A Wack, PA DiMaggio, HJM Brady
    J. Immunol., 2018-01-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Notch Ligand Binding Assay Using Flow Cytometry
    Authors: S Varshney, P Stanley
    Bio Protoc, 2017-12-05;7(23):.
    Species: Human, Mouse
    Sample Types: Recombinant Protein, Whole Cells
    Applications: Bioassay
  9. CD19 regulates ADAM28-mediated Notch2 cleavage to control the differentiation of marginal zone precursors to MZ B cells
    Authors: Y Zhang, G Zhu, H Xiao, X Liu, G Han, G Chen, C Hou, B Shen, Y Li, N Ma, R Wang
    J. Cell. Mol. Med., 2017-07-14;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Selective regulation of Notch ligands during angiogenesis is mediated by vimentin
    Authors: D Antfolk, M Sjöqvist, F Cheng, K Isoniemi, CL Duran, A Rivero-Mul, C Antila, R Niemi, S Landor, CVC Bouten, KJ Bayless, JE Eriksson, CM Sahlgren
    Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. Fringe proteins modulate Notch-ligand cis and trans interactions to specify signaling states.
    Authors: LeBon, Lauren, Lee, Tom V, Sprinzak, David, Jafar-Nejad, Hamed, Elowitz, Michael
    Elife, 2014-09-25;3(0):e02950.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  12. The Atoh1-lineage gives rise to hair cells and supporting cells within the mammalian cochlea.
    Authors: Driver E, Sillers L, Coate T, Rose M, Kelley M
    Dev Biol, 2013-01-11;376(1):86-98.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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Recombinant Mouse DLL1 Fc Chimera Protein, CF
By Anonymous on 10/13/2018
Application: CellProlif
Recombinant Mouse DLL1 Fc Chimera Protein, CF
By Pawel Mazur on 10/21/2016
Application: CellProlif
Cell Proliferation Recombinant Mouse DLL1 Fc Chimera Protein, CF 5026-DL