Recombinant Mouse Draxin Protein

Recombinant Mouse Draxin Protein Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Draxin

Draxin (Dorsal repulsive axon guidance protein) also called neucrin (neural tissue-specific cysteine-rich protein) is a secreted, 58 kDa, presumably glycosylated member of the draxin family of repulsive guidance proteins (1 ‑ 5). In mammals, it is expressed in developing neurons (axons), astroglia, and likely cells of the developing somite (1 ‑ 3). Its expression appears to be limited to the brain and spinal cord (2, 4). Mouse Draxin mRNA encodes 343 amino acids (aa) that include a 25 aa signal sequence and a 318 aa mature, secreted protein containing one potential N-linked glycosylation site followed by a Cys-rich domain (aa 268 ‑ 327). The pattern of cysteines is similar to the second of two cysteine-rich regions in members of the DKK family of Wnt inhibitors (4). Mature mouse Draxin (aa 26 ‑ 343) shares 80%, 94%, 77% and 76% aa identity with mature human, mouse, equine and bovine Draxin, respectively. Draxin is a repulsive guidance molecule that, like DKKs, acts as a Wnt antagonist by binding to LRP6 (4). Draxin is expressed by midline glial cells that act as intermediate guideposts for corpus callosum axons (1). Inactivation of the mouse Draxin gene results in lack of organization of axons into functional tracts or bundles (fasciculation), and failure of the corpus callosum, hippocampal and anterior commissures to form and cross the midline (1). Draxin-deficient mice also show abnormally small hippocampi, especially within the dentate gyrus, where excess apoptosis is detected during early postnatal life (3). In vitro, Draxin blocks migration of chick neural crest cells and inhibits neurite outgrowth from dorsal spinal cord explants and dorsal tectum explants of chick embryo. In vivo, ectopic over-expression inhibits growth and disrupts the routing of commissural axons of chick spinal cord and dorsal tectum axons (1, 2, 5).

Related Research Areas

• Wnt Inhibitors
• Wnt Intracellular Signaling