Recombinant Mouse EGFR Fc Chimera Protein, CF

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1280-ER-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse EGFR Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind recombinant human EGF in a functional ELISA with an estimated Kd of <10 nM.
Source
Mouse myeloma cell line, NS0-derived mouse EGFR protein
Mouse EGFR
(Leu25-Ser647)
Accession # Q9EP98
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
95.9 kDa (monomer)
SDS-PAGE
135-150 kDa, reducing conditions

Product Datasheets

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1280-ER

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1280-ER

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: EGFR

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins. They contain an extracellular ligand binding domain containing two cysteine-rich domains and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). The mouse EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 623 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 540 aa cytoplasmic domain (3). Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse (4-6). Within the ECD, mouse EGFR shares 88% and 93% aa sequence identity with human and rat EGFR, respectively. It shares 44%-48% aa sequence identity with the ECD of mouse ErbB2, ErbB3, and ErbB4. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7-11). EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (12, 13). EGFR is over-expressed in a wide variety of tumors and is the target of several anti-cancer drugs (14).

References
  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Avivi, A. et al. (1991) Oncogene 6:673.
  4. Reiter, J.L. and N.J. Maihle (1996) Nucleic Acids Res. 24:4050.
  5. Reiter J.L. et al. (2001) Genomics 71:1.
  6. Xu, Y.H. et al. (1984) Nature 309:806.
  7. Graus-Porta, D. et al. (1997) EMBO J. 16:1647.
  8. Yarden, Y. et al. (1987) Biochemistry 26:1434.
  9. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
  10. Lemmon, M.A. et al. (1997) EMBO J. 16:281.
  11. Cohen, S. et al. (1982) J. Biol. Chem. 257:1523.
  12. Sibilia, M. and E.F. Wagner (1995) Science 269:234.
  13. Miettinen, P.J. et al. (1995) Nature 376:337.
  14. Roskoski Jr., R. (2004) Biochem. Biophys. Res. Commun. 319:1.
Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Citation for Recombinant Mouse EGFR Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inhibition of Cell Proliferation by an Anti-EGFR Aptamer.
    Authors: Li N, Nguyen HH, Byrom M, Ellington AD
    PLoS ONE, 2011-06-08;6(6):e20299.
    Species: Human
    Sample Types: RNA
    Applications: Binding Assay

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Recombinant Mouse EGF R/ErbB1 Fc Chimera Protein, CF
By Anonymous on 05/18/2016
Application: Binding assay/Protein-protein interaction
Reason for Rating: background is low, signal is good