Recombinant Mouse Fibulin 7 Protein, CF

Catalog #: 8606-FB Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8606-FB-050

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Fibulin 7 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED₅₀ for this effect is 0.7-3.5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Fibulin 7 protein
Gln25-Phe440, with an N-terminal HA Tag

Accession #

Q501P1

N-terminal Sequence
Analysis

Tyr

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

47 kDa

SDS-PAGE

53-63 kDa, reducing conditions

Product Datasheets

8606-FB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8606-FB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Fibulin 7

Fibulin 7, also known as TM14, is an approximately 46 kDa member of the fibulin family of proteins (1, 2). Structurally, Fibulin 7 contains an N-terminal Sushi domain, 3 central tandem EGF modules, and a C-terminal domain homologous to the fibulin module (2). Mouse Fibulin 7 mRNA is highly expressed in teeth, with weaker expression in brain, kidneys, muscles, and bones (2). Mouse Fibulin 7 shares 86% amino acid sequence identity with human Fibulin 7. Fibulin 7 interacts with several extracellular molecules, including Fibronectin, DSPP, and Fibulin 1 (2). A C-terminal fragment of Fibulin 7 was able to bind Human Umbilical Vein Endothelial Cells (HUVECs) and inhibit HUVEC tube formation and vessel sprouting in aortic ring assays, suggesting that Fibulin 7 may have anti-angiogenic activity (3). Fibulin 7 may be important for proper development, since it is included in a chromosomal deletion that is associated with severe cardiac defects and craniofacial anomalies (4).

References