Recombinant Mouse G-CSF Protein Summary
Product Specifications
Val31-Ala208, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
414-CS
| Formulation | Lyophilized from a 0.2 μm filtered solution in Citric Acid and Tween® 20 with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
414-CS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Citric Acid and Tween® 20 with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: G-CSF
G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.
The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.
In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.
Citations for Recombinant Mouse G-CSF Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Phenotypic screening of signaling motifs that efficiently induce cell proliferation
Authors: Umene, K;Nagamune, T;Kawahara, M;
Scientific reports
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages
Authors: K Maruyama, H Kidoya, N Takemura, E Sugisawa, O Takeuchi, T Kondo, MMA Eid, H Tanaka, MM Martino, N Takakura, Y Takayama, S Akira, A Vandenbon, Y Kumagai
Cell Rep, 2020-07-14;32(2):107906.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Neutrophils are essential for induction of vaccine-like effects by antiviral monoclonal antibody immunotherapies
Authors: M Naranjo-Go, J Lambour, M Piechaczyk, M Pelegrin
JCI Insight, 2018-05-03;3(9):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A Bach2-Cebp Gene Regulatory Network for the Commitment of Multipotent Hematopoietic Progenitors
Authors: A Itoh-Nakad, M Matsumoto, H Kato, J Sasaki, Y Uehara, Y Sato, R Ebina-Shib, M Morooka, R Funayama, K Nakayama, K Ochiai, A Muto, K Igarashi
Cell Rep, 2017-03-07;18(10):2401-2414.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Therapeutic Targeting of the G-CSF Receptor Reduces Neutrophil Trafficking and Joint Inflammation in Antibody-Mediated Inflammatory Arthritis
Authors: Arna E Andrews
J. Immunol., 2016-11-02;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Active enhancers are delineated de novo during hematopoiesis, with limited lineage fidelity among specified primary blood cells.
Authors: Luyten A, Zang C, Liu X, Shivdasani R
Genes Dev, 2014-08-15;28(16):1827-39.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Granulocyte-colony stimulating factor attenuates oligomeric amyloid beta neurotoxicity by activation of neprilysin.
Authors: Doi Y, Takeuchi H, Mizoguchi H, Fukumoto K, Horiuchi H, Jin S, Kawanokuchi J, Parajuli B, Sonobe Y, Mizuno T, Suzumura A
PLoS ONE, 2014-07-25;9(7):e103458.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay, ICC -
Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration.
Authors: Fujihara Y, Takato T, Hoshi K
Stem Cells, 2014-05-01;32(5):1208-19.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
G-CSF drives a posttraumatic immune program that protects the host from infection.
Authors: Gardner J, Noel J, Nikolaidis N, Karns R, Aronow B, Ogle C, McCormack F
J Immunol, 2014-01-27;192(5):2405-17.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Aged mice are unable to mount an effective myeloid response to sepsis.
Authors: Nacionales D, Gentile L, Vanzant E, Lopez M, Cuenca A, Cuenca A, Ungaro R, Li Y, Baslanti T, Bihorac A, Moore F, Baker H, Leeuwenburgh C, Moldawer L, Efron P
J Immunol, 2013-12-13;192(2):612-22.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Identification of transcription factors for lineage-specific ESC differentiation.
Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
Stem Cell Reports, 2013-11-27;1(6):545-59.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A soluble form of LMIR5/CD300b amplifies lipopolysaccharide-induced lethal inflammation in sepsis.
J. Immunol., 2012-07-06;189(4):1773-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment.
Authors: Cook BD, Liu S, Evans T
Blood, 2011-04-22;117(24):6489-97.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ESCs and iPSCs.
Authors: Shimoji K, Yuasa S, Onizuka T, Hattori F, Tanaka T, Hara M, Ohno Y, Chen H, Egasgira T, Seki T, Yae K, Koshimizu U, Ogawa S, Fukuda K
Cell Stem Cell, 2010-03-05;6(3):227-37.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
Authors: Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N
Nature, 2007-12-06;450(7171):825-31.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
Stem Cells, 2007-02-01;25(2):490-9.
Species: Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay -
Bone marrow-derived cells require a functional glucose 6-phosphate transporter for normal myeloid functions.
Authors: Kim SY, Nguyen AD, Gao JL, Murphy PM, Mansfield BC, Chou JY
J. Biol. Chem., 2006-08-04;281(39):28794-801.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand.
Authors: Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, Werb Z, Rafii S
Cell, 2002-05-31;109(5):625-37.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense.
Authors: Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, Schwarzenberger P, Oliver P, Huang W, Zhang P, Zhang J, Shellito JE, Bagby GJ, Nelson S, Charrier K, Peschon JJ, Kolls JK
J. Exp. Med., 2001-08-20;194(4):519-27.
Applications: ELISA (Standard)
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