Recombinant Mouse Galectin-3BP/MAC-2BP Protein, CF

Recombinant Mouse Galectin-3BP/MAC-2BP Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Galectin-3BP/MAC-2BP

Galectin-3 binding protein (Galectin-3BP), called CyCAP (cyclophilin C-associated protein) in the mouse, is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Mouse Galectin-3BP is generally considered the paralog of human Galectin-3BP, which is also called MAC-2 binding protein (MAC-2BP), or 90 kDa tumor associated antigen (TAA90K or 90K) (1, 2). However, mouse Galectin-3BP binds cyclophilin C but not Galectin-3 (formerly MAC-2), while the opposite is true of human Galectin-3BP (3). The 577 amino acid (aa), 77 - 95 kDa mouse Galectin-3BP contains an 18 aa signal sequence and four definitive domains (1 - 6). Domain 1 is a group A scavenger receptor domain, domain 2 is a BTB/POZ domain that mediates dimerization, and domain 3 is an IVR domain that is also found following the POZ domain in Drosophila kelch protein (4, 5). Little is known about domain 4 (5, 6). Mature mouse Galectin-3BP shares 88%, 69%, 67% and 65% aa identity with rat, human, dog and cow Galectin-3BP, respectively. The Galectin-3BP dimer interface residues are generally conserved between mouse and human, so linear dimers that occur in humans are also likely in the mouse (5 - 7). Human Galectin-3BP also forms ring-shaped decamers and dodecamers. Mouse Galectin-3BP is widely expressed and modifies innate immune reactions. It is stimulated in macrophages by adhesion, TNF-alpha or IFN-gamma (2). Mice deficient in Galectin-3BP secrete increased amounts of IL-12, TNF-alpha and IFN-gamma and have lower survival in response to endotoxin (8). Galectin-3BP is induced in rat skin fibroblasts, keratinocytes, macrophages and lymphocytes during wound healing, and wounded Galectin-3BP-deficient mice are slow to heal (9). Rat Galectin-3BP is upregulated after cerebral ischemia (10). Ligands for human Galectin-3BP include some other galectins, collagens and integrins, but remain to be tested for mouse Galectin-3BP binding (5 - 7).

Related Research Areas

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