Recombinant Mouse gp130 Fc Chimera Protein, CF

Name: Recombinant Mouse gp130 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 468-MG
Rating: 5 (1 reviews)

Catalog #: 468-MG
8 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
468-MG-100

Recombinant Mouse gp130 Fc Chimera Protein Bioactivity

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All gp130 Proteins and Enzymes

Product Details

Citations (8)

FAQs

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Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse gp130 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha (Catalog # 227-SR). The ED₅₀ for this effect is 0.12-1.2 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse gp130 protein

Mouse gp130 (Gln23-Glu617) Accession # Q6PDI9	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

Q6PDI9

N-terminal Sequence
Analysis

No results obtained: Gln23 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

94 kDa (monomer)

SDS-PAGE

115 kDa, reducing conditions

Product Datasheets

468-MG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

468-MG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse gp130 Fc Chimera (Catalog # 468-MG) inhibits IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha (Catalog # 227-SR). The ED₅₀ for this effect is 0.12-1.2 ng/mL.

Reconstitution Calculator

Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

References

Narazaki, M. et al. (1993) Blood 82:1120.
Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

Long Name

Glycoprotein 130

Entrez Gene IDs

3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)

Alternate Names

CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

Related Research Areas

Citations for Recombinant Mouse gp130 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Microglial inflammation after chronic spinal cord injury is enhanced by reactive astrocytes via the fibronectin/&beta1 integrin pathway
Authors: S Yoshizaki, T Tamaru, M Hara, K Kijima, M Tanaka, DJ Konno, Y Matsumoto, Y Nakashima, S Okada
Journal of Neuroinflammation, 2021-01-06;18(1):12.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Diabetes Induced Alterations in Murine Vitreous Proteome Are Mitigated by IL-6 Trans-Signaling Inhibition
Authors: R Robinson, H Youngblood, H Iyer, J Bloom, TJ Lee, L Chang, Z Lukowski, W Zhi, A Sharma, S Sharma
Invest. Ophthalmol. Vis. Sci., 2020-09-01;61(11):2.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Interleukin-6 trans-signaling inhibition prevents oxidative stress in a mouse model of early diabetic retinopathy
Authors: R Robinson, M Srinivasan, A Shanmugam, A Ward, V Ganapathy, J Bloom, A Sharma, S Sharma
Redox Biol, 2020-05-13;34(0):101574.
Species: Mouse
Sample Types:
Applications: In Vivo
Tumour necrosis factor alpha promotes secretion of 14-3-3? by inducing necroptosis in macrophages
Authors: G Trimova, K Yamagata, S Iwata, S Hirata, T Zhang, F Uemura, M Satoh, N Biln, S Nakayamada, WP Maksymowyc, Y Tanaka
Arthritis Res. Ther., 2020-02-12;22(1):24.
Species: Mouse
Sample Types: Whole Cell
Applications: Cell Culture
Frontline Science: Proliferation of Ly6C(+) monocytes during urinary tract infections is regulated by IL-6 trans-signaling
Authors: A Dixit, J Bottek, AL Beerlage, J Schuettpel, S Thiebes, A Brenzel, C Garbers, S Rose-John, HW Mittrücker, A Squire, DR Engel
J. Leukoc. Biol., 2017-12-28;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Soluble IL-6R expressed by myeloid cells reduces tumor-specific Th1 differentation and drives tumor progression
Authors: H Tsukamoto, K Fujieda, M Hirayama, T Ikeda, A Yuno, K Matsumura, D Fukuma, K Araki, H Mizuta, H Nakayama, S Senju, Y Nishimura
Cancer Res, 2017-02-24;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Interleukin-6 induces S100A9 expression in colonic epithelial cells through STAT3 activation in experimental ulcerative colitis.
Authors: Lee, Min Jeou, Lee, Jin-Ku, Choi, Ji Won, Lee, Chang-Se, Sim, Ji Hyun, Cho, Chung-Hy, Lee, Kwang-Ho, Cho, Ik-Hyun, Chung, Myung-He, Kim, Hang-Rae, Ye, Sang-Kyu
PLoS ONE, 2012-09-04;7(9):e38801.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
Authors: Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA
J. Immunol., 2010-01-18;184(4):2130-9.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo