Recombinant Mouse IFNAB Protein, CF Summary
Product Specifications
Cys24-Glu190
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10151-IF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
Recombinant Mouse IFNAB (Catalog # 10151-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.6-6 pg/mL.
View Larger
2 μg/lane of Recombinant Mouse IFNAB (Catalog # 10151-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17-25 kDa.
Reconstitution Calculator
Background: IFNAB
The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors (1). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFN alpha subtypes and the various IFN alpha share about 80% sequence homology among them (4, 5). The mouse IFNAB consists of 190 amino acids (aa) including a 23 aa signal peptide and a 167 aa IFNAB main chain. It was found that IFNAB plays an important role in the regulation of DNA synthesis in colong-stimulating factor (CSF)-1-stimulated macrophage, as well as mediates the inhibitory efffects of LPS and TNF alpha (6). IFN regulatory factor 2 (IRF-2) controls IFNAB signaling and the absence of its negative regulation selectively affects development of the CD8 alpha - myeloid DC population (7).
- Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
- Fung, K.Y. et al. (2013) Science 339:1088.
- Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
- Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
- van Pesch, V. et al. (2004) J. Virol. 78:8219.
- Hamilton JA, et al. (1996) J Immunol. 156(7):2553.
- Kenya H, et al. (2004) Proc Natl Acad Sci U S A. 101(8):2416.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse IFNAB Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse IFNAB Protein, CF and earn rewards!
Have you used Recombinant Mouse IFNAB Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
