Home / IGFBP-rp1/IGFBP-7 / Recombinant Mouse IGFBP-rp1/IGFBP-7 Protein, CF

Recombinant Mouse IGFBP-rp1/IGFBP-7 Protein, CF

Catalog #: 2120-B7
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2120-B7-025
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Recombinant Mouse IGFBP-rp1/IGFBP-7 Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind 6Ckine/CCL21 in a functional ELISA. Nagakubo, D. et al. (2003) J. Immunol. 171:553. When Recombinant Mouse IGFBP-rp1/IGFBP-7 is immobilized at 500 ng/mL (100 µL/well), the concentration of Recombinant Human CCL21/6Ckine (Catalog # 366-6C) that produces 50% optimal binding response is found to be 1.5-12 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse IGFBP-rp1/IGFBP-7 protein
Ser28-Leu281, with an N-terminal 10-His tag
Accession #
Q61581
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
27.5 kDa
SDS-PAGE
35 kDa, reducing conditions

Product Datasheets

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2120-B7

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2120-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGFBP-rp1/IGFBP-7

Insulin-like growth factor (IGF) binding protein-related protein 1 (IGFBP-rp1), also known as IGF binding protein-7 (IGFBP-7) and Mac25 is a 31 - 37 kDa secreted glycoprotein that belongs to the IGFBP superfamily of molecules (1, 2). Members of this superfamily are characterized by the presence of 10 ‑ 12 conserved cysteines in the first third of the molecule, and the partial conservation of an xCGCCxxC octapeptide motif that exists in an N‑terminal IGFBP domain (1, 3). Mouse IGFBP-7 cDNA encodes a 281 amino acid (aa) precursor protein that contains a putative 25 aa signal peptide and a 256 aa mature region. The mature protein contains an N‑terminal 43 aa IGFBP domain that is followed by a 52 aa Kazal-type serine proteinase inhibitor domain and a 105 aa C-terminal Ig-like C2‑type domain (4, 5). The molecule is known to contain about 4 kDa of carbohydrate and believed to undergo phosphorylation (6). Mature mouse IGFBP-7 is 95% and 91% aa identical to mature rat and human IGFBP-7, respectively. Cells known to express IGFBP-7 include osteoblasts, select skeletal muscle fibers, visceral and vascular smooth muscle cells, breast epithelium, ciliated epithelium, renal tubular epithelium, astrocytes and oligodendroglia, and vascular endothelium in all tissues except brain (7). IGFBP-7 will bind both IGF-I and insulin, albeit at low affinity. When bound to IGF-I, it participates in growth promoting effects (8). Alternatively, IGFBP-7 by itself is an inhibitor of cell proliferation (6). Thus, its function is unclear. It is known to bind heparin, syndecan-1 and chemokines (8, 9). Based on human studies and aa conservation in relevant regions, mouse IGFBP-7 might be be expected to undergo proteolytic cleavage extracellularly (8). This will create an 8 kDa, 72 aa subunit (containing the IGFBP domain) that is disulfide-linked to a 25 kDa, 184 aa C-terminal subunit. Cleavage will reduce IGF‑I bonding activity and the promotion of IGF‑dependent cell proliferation.

References
  1. Hwa, V. et al. (1999) Endocr. Rev. 20:761. 
  2. Kim, H-S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12981. 
  3. Oh, Y. et al. (1996) J. Biol. Chem. 271:30322. 
  4. Kato, M.V. et al. (1996) Oncogene 12:1361. 
  5. Kato, M.V. (2000) Mol. Med. 6:126.
  6. Wilson, H-M. et al. (2002) Cell Growth Differ. 13:205.
  7. Degeorges, A. et al. (2000) J. Histochem. Cytochem. 48:747.
  8. Ahmed, S. et al. (2003) Biochem. Biophys. Res. Commun. 310:612.
  9. Nagakubo, D. et al. (2003) J. Immunol. 171:553.
Long Name
Insulin-like Growth Factor Binding Protein Related Protein 1
Entrez Gene IDs
3490 (Human); 29817 (Mouse)
Alternate Names
angiomodulin; FSTL2; IBP-7; IGFBP7; IGFBP-7; IGFBP-7PGI2-stimulating factor; IGFBP-7v; IGFBPrp1; IGFBP-rp1; insulin-like growth factor binding protein 7; insulin-like growth factor-binding protein 7; MAC25 protein; Mac25; MAC25IGF-binding protein 7; Prostacyclin-stimulating factor; PSF; PSFAGM; TAF; Tumor-derived adhesion factor

Citations for Recombinant Mouse IGFBP-rp1/IGFBP-7 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. IGFBP7 Deletion Promotes Hepatocellular Carcinoma
    Authors: M Akiel, C Guo, X Li, D Rajasekara, RG Mendoza, CL Robertson, N Jariwala, F Yuan, MA Subler, J Windle, DK Garcia, Z Lai, HH Chen, Y Chen, S Giashuddin, PB Fisher, XY Wang, D Sarkar
    Cancer Res., 2017-06-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Effect of insulin-like growth factor-binding protein 7 on steroidogenesis in granulosa cells derived from equine chorionic gonadotropin-primed immature rat ovaries.
    Authors: Tamura K, Matsushita M, Endo A, Kutsukake M, Kogo H
    Biol. Reprod., 2007-05-23;77(3):485-91.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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