Recombinant Mouse IL-28 R alpha/IFN-lambda R1 Fc Chimera, CF
Recombinant Mouse IL-28 R alpha/IFN-lambda R1 Fc Chimera, CF Summary
Product Specifications
| Mouse IL-28 R alpha (Arg21-Ala227) Accession # AAH57856 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5384-MR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-28 R alpha/IFN-lambda R1
IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a type I transmembrane glycoprotein that is cytokine receptor family 2 member 12 (CRF2-12) (1-4). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R (1-4). Each subunit of this receptor can interact with the interferon-like cytokines (type III interferons) IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3) and IL-29 (IFN-lambda 1). Mouse IL 28A and IL-28B share 97% amino acid (aa) identity; rodents do not express IL-29 (1-4). Mouse IL-28 R alpha cDNA encodes a 535 aa protein with a 20 aa signal peptide, a 207 aa extracellular domain (ECD) with a fibronectin type III motif and four potential N-glycosylation sites, a 21 aa transmembrane sequence, and a proline-rich and acidic 287 aa cytoplasmic domain. A 254 aa splice variant of mouse IL 28 R alpha diverges at aa 224 and lacks a transmembrane sequence (3, 5). The mature mouse IL-28 R alpha ECD shares 83%, 67%, 64% and 63% aa sequence identity with rat, human, canine and bovine IL-28 Ra, respectively. Mouse IL-28 R will respond to human IL-28A (6). IL-28 R is constitutively expressed in most tissues, but its ligands are mainly produced by antigen presenting cells in response to viruses and their products (2-6). Signaling through IL-28 R alpha is similar to that of receptors for type I IFNs and involve tyrosine phosphorylation, activation of JAK tyrosine kinases, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1-7). This signaling pathway induces antiviral activity and up-regulates MHC class I antigen expression (2-7). Anti-proliferative activity has also been shown for IL-28/IL-28 R (7).
- Chen, Q. et al. (2006) Vitam. Horm. 74:207.
- Meager, A. et al. (2005) Cytokine 31:109.
- Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
- Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
- Entrez Accession # EDL29971.
- Lasfar, A. et al. (2006) Cancer Res. 66:4468.
- Dumoutier, L. et al. (2004) J. Biol. Chem. 279:32269.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse IL-28 R alpha/IFN-lambda R1 Fc Chimera, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse IL-28 R alpha/IFN-lambda R1 Fc Chimera, CF and earn rewards!
Have you used Recombinant Mouse IL-28 R alpha/IFN-lambda R1 Fc Chimera, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
