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Recombinant Mouse IL-9 Protein

Catalog #: 409-ML
12 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
409-ML-010/CF
409-ML-050/CF

With Carrier

Catalog # Availability Size / Price Qty
409-ML-010
409-ML-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (12)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-9 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.500-5.00 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-9 protein
Gln19-Pro144
Accession #
P15247
N-terminal Sequence
Analysis
No results obtained, sequencing might be blocked: Gln19 is predicted
Predicted Molecular Mass
14.2 kDa
SDS-PAGE
Multiple bands between 16-25 kDa, reducing conditions

Product Datasheets

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409-ML (with carrier)

Product Datasheet
COA
SDS

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409-ML/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

409-ML

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

409-ML/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-9

Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gamma c) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gamma c to form the functional receptor complex. IL-9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4+ T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature mouse IL-9 shares 57% and 74% amino acid sequence identity with human and rat IL-9, respectively (14).

References
  1. Noelle, R.J. and E.C. Nowak (2010) Nat. Rev. Immunol. 10:683.
  2. Goswami, R. and M.H. Kaplan (2011) J. Immunol. 186:3283.
  3. Nowak, E.C. et al. (2009) J. Exp. Med. 206:1653.
  4. Townsend, M.J. et al. (2000) Immunity 13:573.
  5. Leech, M.D. and R.K. Grencis (2006) J. Immunol. 176:2505.
  6. Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
  7. Osterfeld, H. et al. (2010) J. Allergy Clin. Immunol. 125:469.
  8. Jabeen, R. and M.H. Kaplan (2012) Curr. Opin. Immunol. 24:303.
  9. Tan, C. et al. (2010) J. Immunol. 185:6795.
  10. Jager, A. et al. (2009) J. Immunol. 183:7169.
  11. Elyaman, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:12885.
  12. Lu, L.-F. et al. (2006) Nature 442:997.
  13. Stassen, M. et al. (2000) J. Immunol. 164:5549.
  14. Van Snick, J. et al. (1989) J. Exp. Med. 169:363.
Long Name
Interleukin 9
Entrez Gene IDs
3578 (Human); 16198 (Mouse)
Alternate Names
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40

Citations for Recombinant Mouse IL-9 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Helminth alleviates COVID-19-related cytokine storm in an IL-9-dependent way
    Authors: Cao, Z;Wang, J;Liu, X;Liu, Y;Li, F;Liu, M;Chiu, S;Jin, X;
    mBio
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo assay
  2. Deleterious Role of Th9 Cells in Pulmonary Fibrosis
    Authors: KM Deng, XS Yang, Q Luo, YX She, QY Yu, XX Tang
    Cells, 2021-11-17;10(11):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Pathophysiological Roles of Neuro-Immune Interactions between Enteric Neurons and Mucosal Mast Cells in the Gut of Food Allergy Mice
    Authors: T Yashiro, H Ogata, SF Zaidi, J Lee, S Hayashi, T Yamamoto, M Kadowaki
    Cells, 2021-06-23;10(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. IL-9-triggered lncRNA Gm13568 regulates Notch1 in astrocytes through interaction with CBP/P300: contribute to the pathogenesis of experimental autoimmune encephalomyelitis
    Authors: X Liu, F Zhou, W Wang, G Chen, Q Zhang, R Lv, Z Zhao, X Li, Q Yu, JM Meves, H Hua, X Li, X Wang, H Sun, D Gao
    Journal of Neuroinflammation, 2021-05-11;18(1):108.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Host dysbiosis negatively impacts IL-9-producing T-cell differentiation and antitumour immunity
    Authors: RR Almeida, RS Vieira, A Castoldi, FF Terra, ACL Melo, MCC Canesso, L Lemos, M Cipelli, N Rana, MI Hiyane, EL Pearce, FDS Martins, AMC Faria, NOS Câmara
    Br. J. Cancer, 2020-06-05;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  6. IL-33 drives the antitumor effects of dendritic cells via the induction of Tc9 cells
    Authors: N Liu, Y Jiang, J Chen, H Nan, Y Zhao, X Chu, A Wang, D Wang, T Qin, S Gao, Q Yi, Y Yue, S Wang
    Cell. Mol. Immunol., 2018-10-01;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. IL-9-mediated survival of type 2 innate lymphoid cells promotes damage control in helminth-induced lung inflammation.
    Authors: Turner, Jan-Eric, Morrison, Peter J, Wilhelm, Christop, Wilson, Mark, Ahlfors, Helena, Renauld, Jean-Chr, Panzer, Ulf, Helmby, Helena, Stockinger, Brigitta
    J Exp Med, 2013-11-18;210(13):2951-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Robust tumor immunity to melanoma mediated by interleukin-9-producing T cells.
    Nat. Med., 2012-07-08;1248(0):18.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010-09-29;84(24):12703-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. IL-2, -7, and -15, but not thymic stromal lymphopoeitin, redundantly govern CD4+Foxp3+ regulatory T cell development.
    Authors: Vang KB, Yang J, Mahmud SA, Burchill MA, Vegoe AL, Farrar MA
    J. Immunol., 2008-09-01;181(5):3285-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Interleukin-9 induces mucous cell metaplasia independent of inflammation.
    Authors: Reader JR, Hyde DM, Schelegle ES, Aldrich MC, Stoddard AM, McLane MP, Levitt RC, Tepper JS
    Am. J. Respir. Cell Mol. Biol., 2003-06-01;28(6):664-72.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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