Recombinant Mouse Jagged 2 Fc Chimera Protein, CF Summary
Product Specifications
| Mouse Jagged 2 (Met27-Lys1084) Accession # Q9QYE5 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4748-JG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and EDTA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Jagged 2
Mouse Jagged 2 is a 150 kDa (1) member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins, and thereby regulate cell fate determinations during development (2-6). It is a type 1 transmembrane protein that is synthesized as a 1247 amino acid (aa) precursor. It contains a 23 aa signal sequence, a large, 1059 aa extracellular region, a 21 aa transmembrane region, and a short, 144 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats, many of which are also sites of calcium binding, and a cysteine-rich region just proximal to the transmembrane segment (3). Mouse Jagged 2 shares 93% and 90% aa sequence identity with rat and human Jagged 2, respectively. Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (3). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (3). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (3). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, gamma δ T cell lineage differentiation, and the establishment of functional natural killer cell lines (4-6).
- Tsai, S. et al. (2000) Blood, 96:950.
- Shawber, C. et al. (1996) Dev. Biol. 180:370.
- Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
- Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
- DeHart, S. et al. (2005) Blood 105:3521.
- de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.
Citations for Recombinant Mouse Jagged 2 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2
Authors: S Kakuda, RK Lopilato, A Ito, RS Haltiwange
J. Biol. Chem., 2020-08-19;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Notch signaling in mouse blastocyst development and hatching
Authors: MR Batista, P Diniz, A Torres, D Murta, L Lopes-da-C, E Silva
BMC Dev. Biol., 2020-06-02;20(1):9.
Species: Mouse
Sample Types: Embryo
Applications: Bioassay -
Notch1 signaling in NOTCH1-mutated mantle cell lymphoma depends on Delta-Like ligand 4 and is a potential target for specific antibody therapy
Authors: E Silkensted, F Arenas, B Colom-Sanm, S Xargay-Tor, M Higashi, A Giró, V Rodriguez, P Fuentes, WE Aulitzky, H van der Ku, S Beà, ML Toribio, E Campo, M López-Guer, D Colomer
J. Exp. Clin. Cancer Res., 2019-11-01;38(1):446.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Impaired Notch Signaling Leads to a Decrease in p53 Activity and Mitotic Catastrophe in Aged Muscle Stem Cells
Authors: L Liu, GW Charville, TH Cheung, B Yoo, PJ Santos, M Schroeder, TA Rando
Cell Stem Cell, 2018-09-20;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Notch Ligand Binding Assay Using Flow Cytometry
Authors: S Varshney, P Stanley
Bio Protoc, 2017-12-05;7(23):.
Species: Human, Mouse
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay
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