Recombinant Mouse JAM-C Fc Chimera Protein, CF

Catalog #: 1213-J3
5 Citations Datasheet / COA / SDS

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1213-J3-050

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Citations (5)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse JAM-C Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the adhesion of J45.01 human acute lymphoblastic leukemia T lymphocytes on immobilized JAM-2 (VE-JAM)/Fc Chimera. The ED₅₀ for this effect is 0.1-0.5 µg/mL in the presence of 1 x 10⁵ cells/well. Each well was coated with 100 µL of a 0.2 µg/mL solution of rhJAM-2 (VE-JAM)/Fc Chimera.

Source

Mouse myeloma cell line, NS0-derived mouse JAM-C protein

Mouse JAM-C (Val32 - Asn241) Accession # Q9D8B7	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

Q9D8B7

N-terminal Sequence
Analysis

Val32

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50 kDa (monomer)

SDS-PAGE

64 kDa, reducing conditions

Product Datasheets

1213-J3

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1213-J3

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: JAM-C

The family of junctional adhesion molecules (JAM), comprised of at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-C cDNA predicts a 310 amino acid (aa) residue precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, an 18 aa transmembrane domain and a 51 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3). Mouse JAM-C shares 86% aa sequence identity with its human homologue. It also shares approximately 31% and 35% aa sequence homology with mouse JAM-A and JAM-B, respectively (2). Mouse JAM-C is highly expressed during embryogenesis. In adult tissues, mouse JAM-C is restricted to endothelial cells, lymph endothelial cells in the kidney, lymph node and Peyer’s patches where the protein can be localized to the high endothelial venules (3). Although human JAM-C is expressed on human platelets and a subset of leukocytes, mouse JAM-C expression was not detected on any mouse lymphocytes (4). In contrast to human JAM-C which show weak homotypic interactions, mouse JAM-C was reported to exhibit homotypic interactions (3). Mouse JAM-C has also been shown to have heterotypic interaction with JAM-B. It is likely that mouse JAM-C may play a role in lymphocyte transendothelial migration (4).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.

References

Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
Aurrand-Lions, M. et al. (2001) Blood 98:3699.
Aurrand-Lions, M. et al. (2001) J. Biol. Chem. 276:2733.
Johnson-Leger, C. et al. (2002) Blood 100:25793.

Long Name

Junctional Adhesion Molecule C

Entrez Gene IDs

83700 (Human); 83964 (Mouse)

Alternate Names

CD323; JAM-2; JAM3; JAMC; JAM-C; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C

Related Research Areas

Citations for Recombinant Mouse JAM-C Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Junctional Adhesion Molecule-C Mediates the Recruitment of Embryonic-Endothelial Progenitor Cells to the Perivascular Niche during Tumor Angiogenesis
Authors: M Czabanka, LL Petrilli, S Elvers-Hor, K Bieback, B Albert Imh, P Vajkoczy, M Vinci
Int J Mol Sci, 2020-02-11;21(4):.
Species: Mouse
Sample Types: Whole Cell
Applications: Cell Culture
Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis
Authors: V Radulovic, M van der Ga, S Koide, V Sigurdsson, S Lang, S Kaneko, K Miharada
Cell Rep, 2019-06-04;27(10):2826-2836.e5.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis
Authors: E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen
Cell Adh Migr, 2016-04-25;10(4):419-33.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and LIF.
Authors: Su RW, Jia B, Ni H
PLoS ONE, 2012-04-12;7(4):e34325.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow.
Authors: Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M
Blood, 2011-08-25;118(17):4609-19.
Species: Mouse
Sample Types: Whole Cells
Applications: Adhesion Assay, Flow Cytometry