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Recombinant Mouse Lymphotoxin alpha2/beta1 Protein, CF

Catalog #: 1008-LY/CF
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1008-LY-010/CF
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Lymphotoxin alpha2/beta1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 10-40 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Lymphotoxin protein
Leu34-Leu202 (LT alpha ) & Gly136-Gly306 (LT beta )
Accession #
P09225 (LT alpha ) & P41155 (LT beta )
N-terminal Sequence
Analysis
Leu34 (LT alpha ) & Gly136 (LT beta )
Structure / Form
Noncovalently-linked heterotrimer
Predicted Molecular Mass
18.5 kDa (LT alpha ), 18.4 kDa (LT beta )
SDS-PAGE
18-22 kDa, reducing conditions

Product Datasheets

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1008-LY/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1008-LY/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lymphotoxin

Lymphotoxin-alpha (LT alpha ), also known as tumor necrosis factor (TNF) beta, and lymphotoxin-beta (LT beta ) are members of the TNF family. LT alpha cDNA encodes a 202 amino acid secreted soluble protein with a 33 amino acid signal sequence. LT beta cDNA encodes a 306 amino acid type II membrane protein with an N-terminal cytoplasmic domain (residues 1 - 18), a transmembrane region (residues 29 - 48), and an extracellular domain (residues 49 - 306). Secreted LT alpha assembles as a soluble homotrimer, LT alpha 3. In addition, secreted LT alpha also complexes with the membrane associated LT beta to generate two types of heterotrimers, LT alpha 1/ beta 2 and LT alpha 2/ beta 1 (1). The soluble LT alpha 3 binds both TNF RI (p55) and TNF RII (p75). In contrast, the predominant membrane-bound heterotrimer, LT alpha 1/ beta 2, binds only to the lymphotoxin beta receptor (LT beta R). LT alpha 2/ beta 1 is capable of binding LT beta R, TNF RI (p55), and TNF RII (p75). LT plays a role in normal lymphoid organogenesis (2, 3). Transgenic LT alpha knock-out mice exhibited a loss in lymph node development, a change in splenic architecture, and impaired germinal center formation (4). LT is expressed by activated naïve CD4 cells, unpolarized IL-2-secreting effectors, and Th1 effectors. A loss of LT expression and lack of TNF-alpha or TNF-beta secretion is associated with prior exposure to IL-4 and a Th2 phenotype (5).

References
  1. Browning et al. (1993) Cell 72:847.
  2. Ettinger et al. (1996) Proc. Natl. Acad. Sci. USA 93:13102.
  3. Cuff et al. (1998) J. Immunol. 161:6853.
  4. Browning et al. (1997) J. Immunol. 159:3288.
  5. Gramaglia et al. (1999) J. Immunol. 162:1333.
Entrez Gene IDs
4049 (Human); 16992 (Mouse); 102138497 (Cynomolgus Monkey)
Alternate Names
LT; Lymphotoxin alpha; Lymphotoxin; tnfb; TNFSF1; TNLG1E

Citations for Recombinant Mouse Lymphotoxin alpha2/beta1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y
    Biochem. Biophys. Res. Commun., 2009-05-04;384(3):296-300.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. A contrast agent recognizing activated platelets reveals murine cerebral malaria pathology undetectable by conventional MRI.
    Authors: von Zur Muhlen C, Sibson NR, Peter K, Campbell SJ, Wilainam P, Grau GE, Bode C, Choudhury RP, Anthony DC
    J. Clin. Invest., 2008-03-01;118(3):1198-207.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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