Recombinant Mouse Neurturin Protein Summary
Product Specifications
Also measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse GFR alpha ‑2/GDNF R alpha ‑2 Fc Chimera (Catalog # 429-FR) at 1 µg/mL can bind Recombinant Mouse Neurturin with an apparent KD <3.0 nM.
Pro96-Val195
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
477-MN
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
477-MN/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Neurturin
Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. GDNF family proteins are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1-4). Similar to other TGF-beta family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Biologically active Neurturin is a disulfide-linked homodimer of the carboxy-terminal 100 amino acid residues. Mature mouse Neurturin shares 91% amino acid sequence similarity with human Neurturin. Mature Neurturin also shares about 40% similarities with the other three members of the GDNF family ligands (2, 5). Unlike other members of TGF-beta family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFR alpha -1-GFR alpha -4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFR alpha -2 but can also bind GFR alpha -1 at higher concentration (5-8). Neurturin has been shown to promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. Neurturin is expressed in both neuronal and non-neuronal tissues. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems as well as non-neuronal systems (9).
- Lin, L-FH. et al. (1993) Science 260:1130.
- Kotzbauer, P.T. et al. (1996) Nature 384:467.
- Milbrandt, J. et al. (1998) Neuron 20:245.
- Baloh, R.H. et al. (1998) Neuron 21:1291.
- Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
- Baloh, R.H. et al. (1997) Neuron 18:793.
- Jing, S. et al. (1996) Cell 85:1113.
- Jing, S. et al. (1997) J. Biol. Chem. 272:33111.
- Airaksinen, M. and M. Saarma (2002) Nature Review Neuroscience 3:383.
Citations for Recombinant Mouse Neurturin Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The ESCRT-Related ATPase Vps4 Is Modulated by Interferon during Herpes Simplex Virus 1 Infection
Authors: JR Cabrera, R Manivanh, BJ North, DA Leib
MBio, 2019-03-05;10(2):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons
Authors: S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase
Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;114(12):E2486-E2493.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Neuronal Interferon Signaling Is Required for Protection against Herpes Simplex Virus Replication and Pathogenesis.
Authors: Rosato P, Leib D
PLoS Pathog, 2015-07-08;11(7):e1005028.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Intrinsic innate immunity fails to control herpes simplex virus and vesicular stomatitis virus replication in sensory neurons and fibroblasts.
Authors: Rosato P, Leib D
J Virol, 2014-06-18;88(17):9991-10001.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Parasympathetic stimulation improves epithelial organ regeneration.
Authors: Knox, Sarah M, Lombaert, Isabelle, Haddox, Candace, Abrams, Shaun R, Cotrim, Ana, Wilson, Adrian J, Hoffman, Matthew
Nat Commun, 2013-01-01;4(0):1494.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo.
Authors: Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM
J. Neurosci., 2006-08-16;26(33):8588-99.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Expression and function of GDNF family ligands and receptors in the carotid body.
Authors: Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM
Exp. Neurol., 2005-02-01;191(0):S68-79.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
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