Recombinant Mouse P-Selectin/CD62P His-tag Protein, CF

Recombinant Mouse P-Selectin/CD62P His-tag Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: P-Selectin/CD62P

P-Selectin, also known as GMP-140, LECAM-3, PADGEM, and CD62P, is a cell surface glycoprotein of the Selectin family and is expressed by activated platelets and endothelial cells (1-3). Mouse P-Selectin cDNA encodes a 768 amino acid (aa) type I transmembrane protein with a signal peptide, a large extracellular domain (ECD), a transmembrane domain and a short cytoplasmic domain (4). The mature ECD of mouse P-Selectin contains a C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains (5). The mature ECD of mouse P-selectin shares 66% and 89% aa sequence identity with human and rat, respectively. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides (6). P-Selectin binds to P-Selectin glycoprotein ligand-1 (PSGL-1), a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes (7). P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium (8). This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue (1, 3).

Related Research Areas

• Adhesion Molecules in Angiogenesis
• Adhesion Molecules on Endothelial Cells
• Adhesion Molecules on VSMC
• Endothelial Cell Markers
• Lectins
• Platelets
• Regulatory T Cells (Tregs)
• Selectins