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Recombinant Mouse Pentraxin 2/SAP His-tag Protein, CF

Catalog #: 2558-SAB
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2558-SAB-050
Recombinant Mouse Pentraxin 2/SAP His-tag Protein Binding Activity
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Citations (1)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Pentraxin 2/SAP His-tag Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When recombinant Mouse Pentraxin/SAP His-tag (2558-SAB)  is immobilized at 10 μg/mL (100 µL/well), the concentration of Biotinylated recombinant human FC gamma RIIA/CD32a (R167) Protein that produces 50% of the optimal binding response is found to be approximately 0.5-2.5 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Pentraxin 2/SAP protein
Gln21-Asp224, with a C-terminal 10-His tag
Accession #
P12246
N-terminal Sequence
Analysis
No results obtained: Gln21 predicted.
Predicted Molecular Mass
25 kDa
SDS-PAGE
27-35 kDa, under reducing conditions

Product Datasheets

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2558-SAB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2558-SAB

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CaCl2.
Reconstitution Reconstitute at 100 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse Pentraxin 2/SAP His-tag Protein Binding Activity View Larger

When Recombinant Mouse Pentraxin/SAP His-tag (2558-SAB) is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human FC gamma RIIA/CD32a (R167) that produces 50% of the optimal binding response is found to be approximately 0.5-2.5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pentraxin 2/SAP

Pentraxin 2 (also known as Serum Amyoid P Component or SAP) is a secreted glycoprotein that is a universal non-fibrillar component of amyloid deposits. Amyloid is an abnormal extracellular deposit of insoluble protein fibrils that can lead to tissue damage and disease (1-3). Pentraxin 2 belongs to the pentraxin (pentaxin) family, whose members have a characteristic pentagonal discoid arrangement of five non-covalently bound subunits (4). Pentraxin domains contain the consensus sequence, HxCx(S/T)WxS (x = any amino acid), a lectin fold, and two calcium-binding sites (1). They bind to a variety of unrelated molecules in a calcium-dependent lectin-like manner (1, 4, 5). Pentraxin 2 and C-reactive protein (CRP) are members of the classical or short pentraxin subfamily and share 46% amino acid (aa) identity (1). Mouse Pentraxin 2 is the major acute-phase protein whose expression is dependent on complement activation, IL-6 and/or IL-1 beta, while in humans, CRP is the major acute-phase protein (2, 5, 9). Both are produced and secreted by liver hepatocytes and circulate in plasma. The 204 aa mature mouse Pentraxin 2 shares 79% aa identity with rat Pentraxin 2 and 63-68% aa identity with human, guinea pig, golden hamster, porcine, and bovine Pentraxin 2 (2, 5). Amyloid deposits containing Pentraxin 2 are implicated in a diverse range of diseases including Alzheimer’s, prion diseases, type 2 diabetes and various systemic amyloidoses (3, 6, 7). Pentraxin 2 regulates the solubility of amyloid fibrils and protects them from degradation. In addition to its pathogenic role, Pentraxin 2 also has an important physiological function in innate immunity (8). It is an opsonin that interacts with all three types of human Fc gamma receptors that mediate neutrophil phagocytosis (8). Pentraxin 2 has been proposed to bind and sequester a variety of ligands including auto-antigens, apoptotic cells, chromatin, DNA, and micro-organisms (1-3). Pentraxin 2 is also a normal component of basement membranes (1).

References
  1. Garlanda, C. et al. (2005) Annu. Rev. Immunol. 23:337.
  2. Steel, D. and A. Whitehead (1994) Immunol. Today 15:81.
  3. Alexandrescu, A.T. (2005) Protein Sci. 14:1.
  4. Emsley, J. et al. (1994) Nature 367:338.
  5. Mole, J.E. et al. (1988) J. Immunol. 141:3642.
  6. Gillmore, J.D. et al. (2004) Immunology 112:255.
  7. Pepys, M. et al. (2002) Nature 417:254.
  8. Bharadwaj, D. et al. (2001) J. Immunol. 166:6735.
  9. Szalai, A.J. et al. (2000) J. Immunol. 165:1030.
Long Name
Serum Amyloid P Component
Entrez Gene IDs
325 (Human); 20219 (Mouse); 29339 (Rat)
Alternate Names
9.5S alpha-1-glycoprotein; amyloid P component, serum; APCS; MGC88159; Pentraxin 2; PTX2; PTX2serum amyloid P-component; SAP; SAPpentaxin-related; Serum Amyloid P component

Citation for Recombinant Mouse Pentraxin 2/SAP His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Regulatory T cells function in established systemic inflammation and reverse fatal autoimmunity
    Authors: W Hu, ZM Wang, Y Feng, M Schizas, BE Hoyos, J van der Ve, JG Verter, R Bou-Puerto, AY Rudensky
    Nature Immunology, 2021-08-23;22(9):1163-1174.
    Species: Mouse
    Sample Types: Reference Standard

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