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Recombinant Mouse Plexin B2 Protein, CF

Catalog #: 6836-PB Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
6836-PB-050
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Plexin B2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When Recombinant Mouse Plexin B2 is coated at 5 μg/mL, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera (Catalog # 5235-S4) binds with an apparent KD < 10 nM. 

Optimal dilutions should be determined by each laboratory for each applications.

Source
Chinese Hamster Ovary cell line, CHO-derived mouse Plexin B2 protein
Mouse Plexin B2
(Leu20 - Trp1029)
Accession # NP_001152993		 HPGGGSGGGSGGGS 6-His tag
N-terminus C-terminus
Accession #
NP_001152993
N-terminal Sequence
Analysis
Leu20
Predicted Molecular Mass
112 kDa
SDS-PAGE
135-155 kDa, reducing conditions

Product Datasheets

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6836-PB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6836-PB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Plexin B2

Plexin B2 is a 240 kDa type I transmembrane (TM) glycoprotein of the Plexin B family of semaphorin receptors (1, 2). The mouse Plexin B2 cDNA encodes 1842 amino acids (aa) that include a 19 aa signal sequence, a 1182 aa extracellular domain (ECD), a 21 aa TM domain, and a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20 ‑ 468) and three IPT repeats (aa 806 ‑ 1096). The ECD may be cleaved into two subunits, a 170 kDa alpha ‑chain (aa 20 ‑ 1168) and an 80 kDa TM beta ‑chain, that remain noncovalently linked (1). Multiple splice variants may exist. Within aa 20 ‑ 1029 in the ECD, mouse Plexin B2 shares 82%, 93%, 80% and 79% aa identity with human, rat, canine and bovine Plexin B2, respectively. The B Plexins (B1, B2 and B3) share approximately 40% aa identity with each other. Plexin B2 mRNA is expressed in proliferating cerebellar granule cell progenitors, neuroepithelium, developing neurons, growth plate chondrocytes, tooth bud inner enamel epithelium, glomeruli and mesenchyme of the developing kidney, and in germinal center B lymphocytes when T cell help is present (3 ‑ 7). Plexin B2 is often co‑expressed with Plexin B1, and the two may form heterodimers (1, 4, 6). Genetic deletion of mouse Plexin B2 results in defects in proliferation and migration of cerebellar granule cells, abnormal development of the neural tube and disorganization of the embryonic brain; these defects are not seen when Plexin B1 is deleted (8 - 10). In adults, Plexin B2 is expressed in specialized vascular endothelia, pancreatic islets of Langerhans, and adrenal glands (11). Plexin B2 serves as a receptor for type 4 semaphorins, especially Sema4C and Sema4G (8 ‑ 12). B Plexins, including Plexin B2, can bind the scatter factor receptors, Met and Ron, and activate them upon semaphorin engagement (1, 13).

References
  1. Artigiani, S. et al. (2003) J. Biol. Chem. 278:10094.
  2. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
  3. Friedel, R.H. et al. (2007) J. Neurosci. 27:3921.
  4. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  5. Zhang, M. et al. (2008) Bone 43:511.
  6. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355.
  7. Yu, D. et al. (2008) Immunol. Cell Biol. 86:3.
  8. Deng, S. et al. (2007) J. Neurosci. 27:6333.
  9. Hirschberg, A. et al. (2010) Mol. Cell. Biol. 30:764.
  10. Maier, V. et al. (2011) Mol. Cell. Neurosci. 46:419.
  11. Zielonka, M. et al. (2010) Exp. Cell Res. 316:2477.
  12. Yukawa, K. et al. (2010) Int. J. Mol. Med. 25:225.
  13. Conrotto, P. et al. (2004) Oncogene 23:5131.
Entrez Gene IDs
23654 (Human); 140570 (Mouse); 315217 (Rat)
Alternate Names
MM1; MM1KIAA0315dJ402G11.3; Nbla00445; PLEXB2; Plexin B2; plexin-B2; PLXNB2

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