Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein
Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein Summary
Product Specifications
Met1-Gln265
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7150-RS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
7150-RS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (1324-WN).
Reconstitution Calculator
Background: R-Spondin 1
R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).
Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Mouse R‑Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21‑209.
- Chen, J.-Z. et al. (2002) Mol. Biol. Rep. 29:287.
- Kim, K.-A. et al. (2006) Cell Cycle 5:23.
- Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
- Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
- Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
- Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
- Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
- Parma, P. et al. (2006) Nat. Genet. 38:1304.
- Kim, K.-A. et al. (2005) Science 309:1256.
- Zhao, J. et al. (2007) Gastroenterology 132:1331.
- Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
- Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
- Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
Citations for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis
Authors: A Hiroto, WK Kim, A Pineda, Y He, DH Lee, V Le, AW Olson, J Aldahl, CH Nenninger, AJ Buckley, GQ Xiao, J Geradts, Z Sun
Nature Communications, 2022-11-02;13(1):6552.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Mitochondrial transcription factor A in RORgammat+ lymphocytes regulate small intestine homeostasis and metabolism
Authors: Z Fu, JW Dean, L Xiong, MW Dougherty, KN Oliff, ZE Chen, C Jobin, TJ Garrett, L Zhou
Nature Communications, 2021-07-22;12(1):4462.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1
Authors: K Dunbar, A Valanciute, A Lima, PF Vinuela, T Jamieson, V Rajasekara, J Blackmur, AM Ochocka-Fo, A Guazzelli, P Cammareri, MJ Arends, OJ Sansom, KB Myant, SM Farrington, MG Dunlop, FVN Din
Cell Mol Gastroenterol Hepatol, 2020-09-22;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: Cell Culture -
Gut microbiota maturation during early human life induces enterocyte proliferation via microbial metabolites
Authors: MW Dougherty, O Kudin, M Mühlbauer, J Neu, RZ Gharaibeh, C Jobin
BMC Microbiol., 2020-07-11;20(1):205.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay -
Indispensable role of the Ubiquitin-fold modifier 1-specific E3 ligase in maintaining intestinal homeostasis and controlling gut inflammation
Authors: Y Cai, G Zhu, S Liu, Z Pan, M Quintero, CJ Poole, C Lu, H Zhu, B Islam, JV Riggelen, D Browning, K Liu, R Blumberg, N Singh, H Li
Cell Discov, 2019-01-29;5(0):7.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Essential role of IFN-? in T cell-associated intestinal inflammation
Authors: Y Eriguchi, K Nakamura, Y Yokoi, R Sugimoto, S Takahashi, D Hashimoto, T Teshima, T Ayabe, ME Selsted, AJ Ouellette
JCI Insight, 2018-09-20;3(18):.
Species: Mouse
Sample Types: Intestinal organoids
Applications: Bioassay -
PDGFR? + pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo
Authors: G Greicius, Z Kabiri, K Sigmundsso, C Liang, R Bunte, MK Singh, DM Virshup
Proc. Natl. Acad. Sci. U.S.A., 2018-03-20;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
RSPOs facilitated HSC activation and promoted hepatic fibrogenesis
Authors: X Yin, H Yi, L Wang, W Wu, X Wu, L Yu
Oncotarget, 2016-09-27;7(39):63767-63778.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage
Authors: Marie-Isabelle Garcia
Development, 2016-03-17;143(9):1452-63.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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What are the differences between Recombinant Mouse R-Spondin 1 Protein, CF (Catalog # 3474-RS) and the new version, Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein (Catalog # 7150-RS)?
Catalog # 3474-RS is an E. coli cell derived R-Spondin 1 protein with aaSer21-Gly209. The bioactivity is measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a. Additionally, the purity specification for this protein is >90%.
Catalog # 7150-RS is a Chinese Hamster Ovary (CHO) cell derived R-Spondin 1 protein with aaSer21-Gln265. The bioactivity is measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt‑3a. Additionally, the purity specification for this protein is >95%.
Reviews for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein
Average Rating: 4.7 (Based on 3 Reviews)
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