Recombinant Mouse Resistin Protein Summary
Product Specifications
Ser21-Ser114
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1069-RN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1069-RN/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Resistin
Resistin, also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a member of a family of secreted cysteine-rich peptide hormones that also includes Resistin-like molecules RELM alpha, beta, and gamma. These molecules play important roles in inflammation, glucose metabolism, and insulin resistance (1, 2). Mature mouse Resistin is a 12 kDa protein with an N-terminal alpha -helical domain and a C-terminal beta -sandwich domain that is stabilized by multiple intrachain disulfide bonds (3). Resistin circulates as noncovalent trimers and disulfide-linked hexamers, with the trimeric form showing greater bioactivity (3, 4). Resistin can also form multimers with RELM beta (4). Mature mouse Resistin shares 56% and 72% amino acid (aa) sequence identity with human and rat Resistin, respectively. It shares 34% - 42% aa sequence identity with mouse RELM alpha, beta, and gamma. In rodents, Resistin is expressed by adipocytes and in the pituitary and arcuate nucleus of the hypothalamus (5 - 9). It is upregulated during adipogenesis, in obesity, and by insulin or a high carbohydrate diet (5, 6). This is in contrast to human Resistin which is produced by macrophages and monocytes but not by adipocytes (9 - 12). Mouse Resistin induces proinflammatory molecule production in adipocytes and promotes hepatic gluconeogenesis and insulin resistance (5, 7). Human Resistin promotes lipolysis by human and mouse adipocytes, but mouse Resistin does not promote lipolysis by adipocytes of either species (10). Both mouse and human Resistin promote vascular endothelial cell sprouting in vitro and inflammatory reactions in vivo (13 - 16).
- Adeghate, E. (2004) Cell. Mol. Life Sci. 61:2485.
- Asano, T. et al. (2006) Curr. Diabetes Rev. 2:449.
- Patel, S.D. et al. (2004) Science 304:1154.
- Chen, J. et al. (2002) J. Endocrinol. 175:499.
- Steppan, C.M. et al. (2001) Nature 409:307.
- Kim, K-H. et al. (2001) J. Biol. Chem. 276:11252.
- Banerjee, R.R. et al. (2004) Science 303:1195.
- Rajala, M.W. et al. (2002) Mol. Endocrinol. 16:1920.
- Morash, B.A. et al. (2002) FEBS Lett. 526:26.
- Ort, T. et al. (2005) Endocrinology 146:2200.
- Nagaev, I. et al. (2006) Plos ONE 1:e31.
- Savage, D.B. et al. (2001) Diabetes 50:2199.
- Mu, H. et al. (2006) Cardiovasc. Res. 70:146.
- Robertson, S.A. et al. (2008) Regul. Pept. August 5 epub.
- Bokarewa, M. et al. (2005) J. Immunol. 174:5789.
- Burnett, M.S. et al. (2005) Atherosclerosis 182:241.
Citations for Recombinant Mouse Resistin Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions.
Authors: Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S
PLoS ONE, 2015-01-28;10(1):e0117908.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Inhibition of adipocyte differentiation by resistin-like molecule alpha. Biochemical characterization of its oligomeric nature.
Authors: Blagoev B, Kratchmarova I, Nielsen MM, Fernandez MM, Voldby J, Andersen JS, Kristiansen K, Pandey A, Mann M
J. Biol. Chem., 2002-08-19;277(44):42011-6.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse Resistin Protein
There are currently no reviews for this product. Be the first to review Recombinant Mouse Resistin Protein and earn rewards!
Have you used Recombinant Mouse Resistin Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
