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Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein

Catalog #: 461-SH
36 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
461-SH-025/CF

With Carrier

Catalog # Availability Size / Price Qty
461-SH-025
Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein Bioactivity
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Citations (36)
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Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.6-3 µg/mL.
Source
E. coli-derived mouse Sonic Hedgehog/Shh protein
Cys25-Gly198, with a C-terminal 6-His tag
Accession #
Q62226
N-terminal Sequence
Analysis
Cys25
Predicted Molecular Mass
20 kDa

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461-SH (with carrier)

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461-SH/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

461-SH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

461-SH/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein Bioactivity View Larger

Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus (Catalog # 461-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.6-3 μg/mL.

SDS-PAGE Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Mouse Shh encodes a 437 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).

References
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Echelard, Y. et al. (1993) Cell 75:1417.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.
Entrez Gene IDs
6469 (Human); 20423 (Mouse)
Alternate Names
HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

Citations for Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Effect of Sonic Hedgehog on the Regeneration of Epidermal Texture Patterns
    Authors: K Takaya, N Aramaki-Ha, S Sakai, K Okabe, K Kishi
    Biomedicines, 2022-12-01;10(12):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Forebrain Shh overexpression improves cognitive function and locomotor hyperactivity in an aneuploid mouse model of Down syndrome and its euploid littermates
    Authors: FJ Gao, D Klinedinst, FX Fernandez, B Cheng, A Savonenko, B Devenney, Y Li, D Wu, MG Pomper, RH Reeves
    Acta neuropathologica communications, 2021-08-16;9(1):137.
    Applications: MALDI-TOF, Western Blot
  3. BMI1 regulates multiple myeloma-associated macrophage's pro-myeloma functions
    Authors: D Zhang, J Huang, F Wang, H Ding, Y Cui, Y Yang, J Xu, H Luo, Y Gao, L Pan, Y Wu, Y Gong, L Xie, Z Liu, Y Qu, L Zhang, W Liu, W Zhang, S Zhao, Q Yi, T Niu, Y Zheng
    Cell Death & Disease, 2021-05-15;12(5):495.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. PDGFRA in vascular adventitial MSCs promotes neointima formation in arteriovenous fistula in chronic kidney disease
    Authors: K Song, Y Qing, Q Guo, EK Peden, C Chen, WE Mitch, L Truong, J Cheng
    JCI Insight, 2020-11-05;0(0):.
    Species: Mouse
    Sample Types: Virus
    Applications: Bioassay
  5. SMO-M2 mutation does not support cell-autonomous Hedgehog activity in cerebellar granule cell precursors
    Authors: M Petroni, M Sahùn Ronc, V Ramponi, F Fabretti, V Nicolis Di, M Moretti, V Alfano, A Corsi, S De Panfili, M Giubettini, S Di Giulio, C Capalbo, F Belardinil, A Coppa, F Sardina, V Colicchia, F Pedretti, P Infante, B Cardinali, A Tessitore, G Canettieri, E De Smaele, G Giannini
    Sci Rep, 2019-12-23;9(1):19623.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Hedgehog signaling promotes tumor-associated macrophage polarization to suppress intratumoral CD8+ T cell recruitment
    Authors: AJ Petty, A Li, X Wang, R Dai, B Heyman, D Hsu, X Huang, Y Yang
    J. Clin. Invest., 2019-12-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors
    Authors: M Matjusaiti, LJ Wagstaff, A Martella, B Baranowski, C Blin, S Gogolok, A Williams, SM Pollard
    Stem Cell Reports, 2019-11-07;13(6):1053-1067.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Ciliary IFT80 regulates dental pulp stem cells differentiation by FGF/FGFR1 and Hh/BMP2 signaling
    Authors: X Yuan, M Liu, X Cao, S Yang
    Int. J. Biol. Sci., 2019-08-06;15(10):2087-2099.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. High expression of Sonic hedgehog in allergic airway epithelia contributes to goblet cell metaplasia
    Authors: C Xu, C Zou, M Hussain, W Shi, Y Shao, Z Jiang, X Wu, M Lu, J Wu, Q Xie, Y Ke, F Long, L Tang, X Wu
    Mucosal Immunol, 2018-06-04;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Directed transdifferentiation of M�ller glial cells to photoreceptors using the sonic hedgehog signaling pathway agonist purmorphamine
    Authors: D Gu, S Wang, S Zhang, P Zhang, G Zhou
    Mol Med Rep, 2017-09-28;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  11. The transcription factor Gli3 promotes B cell development in fetal liver through repression of Shh
    Authors: A Solanki, CI Lau, JI Saldaña, S Ross, T Crompton
    J. Exp. Med., 2017-05-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  12. Deficiency of NOX1 or NOX4 Prevents Liver Inflammation and Fibrosis in Mice through Inhibition of Hepatic Stellate Cell Activation.
    Authors: Lan T, Kisseleva T, Brenner D
    PLoS ONE, 2015-07-29;10(7):e0129743.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Modulation of Ciliary Phosphoinositide Content Regulates Trafficking and Sonic Hedgehog Signaling Output.
    Authors: Chavez M, Ena S, Van Sande J, de Kerchove d'Exaerde A, Schurmans S, Schiffmann S
    Dev Cell, 2015-07-16;34(3):338-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Deletion of IFT80 Impairs Epiphyseal and Articular Cartilage Formation Due to Disruption of Chondrocyte Differentiation.
    Authors: Yuan X, Yang S
    PLoS ONE, 2015-06-22;10(6):e0130618.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions.
    Authors: Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S
    PLoS ONE, 2015-01-28;10(1):e0117908.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Generation of rat-induced pluripotent stem cells from a new model of metabolic syndrome.
    Authors: Takenaka-Ninagawa, Nana, Kawabata, Yuka, Watanabe, Shogo, Nagata, Kohzo, Torihashi, Shigeko
    PLoS ONE, 2014-08-11;9(8):e104462.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  17. The fate of the primary cilium during myofibroblast transition.
    Authors: Rozycki M, Lodyga M, Lam J, Miranda M, Fatyol K, Speight P, Kapus A
    Mol Biol Cell, 2014-01-08;25(5):643-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. A modular gain-of-function approach to generate cortical interneuron subtypes from ES cells.
    Authors: Au E, Ahmed T, Karayannis T, Biswas S, Gan L, Fishell G
    Neuron, 2013-12-04;80(5):1145-58.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. The acid-secreting parietal cell as an endocrine source of Sonic Hedgehog during gastric repair.
    Authors: Engevik A, Feng R, Yang L, Zavros Y
    Endocrinology, 2013-10-03;154(12):4627-39.
    Species: Mouse
    Sample Types: In Vivo
  20. MicroRNA-17-92 cluster mediates the proliferation and survival of neural progenitor cells after stroke.
    Authors: Liu, Xian Shu, Chopp, Michael, Wang, Xin Li, Zhang, Li, Hozeska-Solgot, Ann, Tang, Tao, Kassis, Haifa, Zhang, Rui Lan, Chen, Charles, Xu, Jennifer, Zhang, Zheng Ga
    J Biol Chem, 2013-03-19;288(18):12478-88.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: In Vivo
  21. Loss of the transcription factor GLI1 identifies a signaling network in the tumor microenvironment mediating KRAS oncogene-induced transformation.
    Authors: Mills, Lisa D, Zhang, Yaqing, Marler, Ronald J, Herreros-Villanueva, Marta, Zhang, Lizhi, Almada, Luciana, Couch, Fergus, Wetmore, Cynthia, Pasca di Magliano, Marina, Fernandez-Zapico, Martin E
    J Biol Chem, 2013-03-12;288(17):11786-94.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  22. Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord.
    Authors: Belgacem YH, Borodinsky LN
    Proc. Natl. Acad. Sci. U.S.A., 2011-02-28;108(11):4482-7.
    Species: Xenopus
    Sample Types: Whole Tissue
    Applications: Bioassay
  23. Notch and Wnt signaling mediated rod photoreceptor regeneration by Muller cells in adult mammalian retina.
    Authors: Del Debbio CB, Balasubramanian S, Parameswaran S, Chaudhuri A, Qiu F, Ahmad I
    PLoS ONE, 2010-08-26;5(8):e12425.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  24. Establishment and characterization of immortalized Gli-null mouse embryonic fibroblast cell lines.
    Authors: Lipinski RJ, Bijlsma MF, Gipp JJ, Podhaizer DJ, Bushman W
    BMC Cell Biol., 2008-09-13;9(0):49.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Sonic hedgehog promotes stem-cell potential of Muller glia in the mammalian retina.
    Authors: Wan J, Zheng H, Xiao HL, She ZJ, Zhou GM
    Biochem. Biophys. Res. Commun., 2007-09-10;363(2):347-54.
    Species: Rat
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  26. Differentiation of ES cells into cerebellar neurons.
    Authors: Salero E, Hatten ME
    Proc. Natl. Acad. Sci. U.S.A., 2007-02-09;104(8):2997-3002.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Regulation of cavernous nerve injury-induced apoptosis by sonic hedgehog.
    Authors: Podlasek CA, Meroz CL, Tang Y, McKenna KE, McVary KT
    Biol. Reprod., 2006-09-20;76(1):19-28.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  28. An early role for sonic hedgehog from foregut endoderm in jaw development: ensuring neural crest cell survival.
    Authors: Brito JM, Teillet MA, Le Douarin NM
    Proc. Natl. Acad. Sci. U.S.A., 2006-07-25;103(31):11607-12.
    Species: Avian - Quail, Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  29. Expression of the short stature homeobox gene Shox is restricted by proximal and distal signals in chick limb buds and affects the length of skeletal elements.
    Authors: Tiecke E, Bangs F, Blaschke R, Farrell ER, Rappold G, Tickle C
    Dev. Biol., 2006-07-12;298(2):585-96.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo
  30. Embryonic stem cell-derived neuron models of Parkinson's disease exhibit delayed neuronal death.
    Authors: Yamashita H, Nakamura T, Takahashi T, Nagano Y, Hiji M, Hirabayashi T, Amano T, Yagi T, Sakai N, Kohriyama T, Matsumoto M
    J. Neurochem., 2006-07-01;98(1):45-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Multipotentiality, homing properties, and pyramidal neurogenesis of CNS-derived LeX(ssea-1)+/CXCR4+ stem cells.
    Authors: Corti S, Locatelli F, Papadimitriou D, Donadoni C, Del Bo R, Fortunato F, Strazzer S, Salani S, Bresolin N, Comi GP
    FASEB J., 2005-09-08;19(13):1860-2.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Shh and Fgf8 act synergistically to drive cartilage outgrowth during cranial development.
    Authors: Abzhanov A, Tabin CJ
    Dev. Biol., 2004-09-01;273(1):134-48.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  33. Derivation of midbrain dopamine neurons from human embryonic stem cells.
    Authors: Perrier AL, Tabar V, Barberi T, Rubio ME, Bruses J, Topf N, Harrison NL, Studer L
    Proc. Natl. Acad. Sci. U.S.A., 2004-08-13;101(34):12543-8.
    Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  34. FGF8 dose-dependent regulation of embryonic submandibular salivary gland morphogenesis.
    Authors: Jaskoll T, Witcher D, Toreno L, Bringas P, Moon AM, Melnick M
    Dev. Biol., 2004-04-15;268(2):457-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2.
    Authors: Viti J, Gulacsi A, Lillien L
    J. Neurosci., 2003-07-02;23(13):5919-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  36. Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium.
    Authors: Mistretta CM, Liu HX, Gaffield W, MacCallum DK
    Dev. Biol., 2003-02-01;254(1):1-18.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the difference between Recombinant Mouse Sonic Hedgehog Catalog # 464-SH and Catalog # 461-SH?

    • Recombinant Mouse Sonic Hedgehog, Catalog # 464-SH, possesses a N-terminal mutation that increases its potency in bioassay tests. The amino acid sequence is Cys25-Gly198 (Cys25Ile-Ile), accession number Q62226. The Cys25Ile-Ile mutation was created to match a publication that describes enhanced activity with these modifications: "http://www.ncbi.nlm.nih.gov/pubmed/11284692". Catalog # 461-SH does not possess this mutation.

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