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Recombinant Mouse ST2/IL-33R His-tag Protein, CF

Catalog #: 10695-MR Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10695-MR-050
Recombinant Mouse ST2/IL-33R His-tag Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse ST2/IL-33R His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse ST2/IL-33R His-tag (Catalog # 10695-MR) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Mouse IL-33  Recombinant Mouse IL‑33 (Catalog # 3626-ML) binds with an ED50 of 0.03-0.27 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse ST2/IL-33R protein
Ser27-His331, with a C-terminal 6-His tag
Accession #
P14719.2
N-terminal Sequence
Analysis
Ser27
Predicted Molecular Mass
36 kDa
SDS-PAGE
57-68 kDa, under reducing conditions

Product Datasheets

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10695-MR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10695-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Mouse ST2/IL-33R His-tag (Catalog # 10695-MR) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Mouse IL-33 (3626-ML) binds with an ED50 of 0.03-0.27 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse ST2/IL-33R His-tag (Catalog # 10695-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-68 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ST2/IL-33R

ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 59% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is believed to be a useful biomarker for predicting both acute and chronic heart failure, and is expressed mainly in two isoforms, as a transmembrane (ST2L) and as a soluble form (sST2) (4,5). ST2L is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (6-9). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (6, 10). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 11, 12). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (8). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (6, 13-15). ST2 is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (7, 9, 16). Level of ST2 expression is associated with gastric cancer progression (17). When bound to IL-33, ST2 is indicated as an immunotherapy target for diagnosis and treament of cancer patients likely to be resistant to conventional treatments (18). Soluble ST2 functions as a decoy receptor that blocks IL-33's ability to signal through transmembrane ST2 (11, 14-16).

References
  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.
  4. Pascual-Figal DA et al. (2015) Am J Cardiol 115(7 Suppl):3B-7B.
  5. Villacorta, Humberto et al. (2016) Arquivos brasileiros de cardiologia vol. 106,2.
  6. Schmitz, J. et al. (2005) Immunity 23:479.
  7. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  8. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  9. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  10. Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
  11. Palmer, G. et al. (2008) Cytokine 42:358.
  12. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  13. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  14. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  15. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  16. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
  17. Bai, F. et al. (2019) Oncol Lett. 17:5761.
  18. Kudo-Saito, C. et al. (2020) Cancer Res. 80:1981.
Long Name
Interleukin 33 Receptor
Entrez Gene IDs
9173 (Human); 17082 (Mouse)
Alternate Names
DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed

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