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Recombinant Mouse Thrombospondin-1 Protein, CF

Catalog #: 7859-TH
10 Citations Datasheet / COA / SDS
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7859-TH-050
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Citations (10)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Thrombospondin-1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 0.1-0.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Thrombospondin-1 protein
Mouse Thrombospondin-1
(Asn19-Ser1171)
Accession # NP_035710		 GGGSGGGSGGGS 6-His tag
N-terminus C-terminus
Accession #
NP_035710
N-terminal Sequence
Analysis
Starts at Asn19
Predicted Molecular Mass
129 kDa
SDS-PAGE
130-155 kDa, reducing conditions

Product Datasheets

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7859-TH

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7859-TH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombospondin-1

Thrombospondin‑1 (TSP‑1) is a 150‑180 kDa calcium‑sensitive protein that is secreted as a disulfide‑linked homotrimer. Within the Thrombospondin family, TSP‑2 is also homotrimeric, while TSP-3, -4, and -5/COMP are pentameric. TSP‑1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N‑terminal Laminin G‑like globular domain, an extended central region with one vWF‑C domain, 3 TSP type 1 repeats, 3 EGF‑like domains, and 8 TSP type‑3 repeats, and a globular C‑terminal lectin domain. Distinct regions of TSP‑1 have been associated with binding to particular ECM or cellular molecules (1, 2). Mature mouse TSP‑1 shares 95% and 99% amino acid sequence identity with human and rat TSP‑1, respectively. TSP‑1 counteracts the angiogenic, hypotensive, and anti‑thrombotic effects of nitric oxide (NO) (3). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC (4‑6). TSP‑1’s actions on vascular smooth muscle cells support arterial tone and blood pressure in the presence of vasodilatory stimulation (7). It is released from activated platelets and enhances platelet aggregation and adhesion (8). TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF‑beta and inducing release of the active cytokine from the latency associated peptide (LAP) (9, 10). TSP‑1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling (11, 12). In the nervous system, it promotes excitatory synapse formation (13, 14) and supports the integration of neural progenitor cells into the olfactory bulb (15). In addition, TSP‑1 is released by apoptotic cells and promotes macrophage-mediated debris clearance (16).

References
  1. Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152.
  2. Laherty, C.D. et al. (1992) J. Biol. Chem. 267:3274.
  3. Roberts, D.D. et al. (2012) Matrix Biol. 31:162.
  4. Gupta, K. et al. (1999) Angiogenesis 3:147.
  5. Kaur, S. et al. (2010) J. Biol. Chem. 285:38923.
  6. Garg, P. et al. (2011) Am. J. Physiol. Lung Cell. Mol. Physiol. 301:L79.
  7. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
  8. Isenberg, J.S. et al. (2008) Blood 111:613.
  9. Schultz-Cherry, S. et al. (1994) J. Biol. Chem. 269:26775.
  10. Sweetwyne, M.T. and J.E. Murphy-Ullrich (2012) Matrix Biol. 31:178.
  11. Grimbert, P. et al. (2006) J. Immunol. 177:3534.
  12. Kaur, S. et al. (2011) J. Biol. Chem. 286:14991.
  13. Xu, J. et al. (2010) Nat. Neurosci. 13:22.
  14. Eroglu, C. et al. (2009) Cell 139:380.
  15. Blake, S.M. et al. (2008) EMBO J. 27:3069.
  16. Moodley, Y. et al. (2003) Am. J. Pathol. 162:771.
Entrez Gene IDs
7057 (Human); 21825 (Mouse)
Alternate Names
THBS; THBS1; THBS-1; thrombospondin 1; Thrombospondin1; Thrombospondin-1; thrombospondin-1p180; TSP-1; TSP1thrombospondin-1; TSPTSP-1

Citations for Recombinant Mouse Thrombospondin-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Pro-inflammatory macrophages impair skeletal muscle regeneration in ischemic-damaged limbs by inducing precocious differentiation of satellite cells
    Authors: KW Southerlan, Y Xu, DT Peters, X Wei, X Lin, Y Xiang, K Fei, LA Olivere, JM Morowitz, J Otto, Q Dai, CD Kontos, Y Diao
    bioRxiv : the preprint server for biology, 2023-04-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme
    Authors: G Jacquemin, A Wurmser, M Huyghe, W Sun, Z Homayed, C Merle, M Perkins, F Qasrawi, S Richon, F Dingli, G Arras, D Loew, D Vignjevic, J Pannequin, S Fre
    Elife, 2022-05-11;11(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. mTOR inhibition prevents angiotensin II-induced aortic rupture and pseudoaneurysm but promotes dissection in Apoe-deficient mice
    Authors: C He, B Jiang, M Wang, P Ren, SI Murtada, AW Caulk, G Li, L Qin, R Assi, CJ Lovoulos, MA Schwartz, JD Humphrey, G Tellides
    JCI Insight, 2022-02-08;7(3):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Targeting Ovarian Carcinoma with TSP-1:CD47 Antagonist TAX2 Activates Anti-Tumor Immunity
    Authors: A Jeanne, T Sarazin, M Charlé, C Moali, C Fichel, C Boulagnon-, M Callewaert, MC Andry, E Diesis, F Delolme, D Rioult, S Dedieu
    Cancers, 2021-10-07;13(19):.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  5. Insulin inhibits inflammation-induced cone death in retinal detachment
    Authors: JB Conart, G Blot, S Augustin, G Millet-Pue, C Roubeix, F Beguier, H Charles-Me, S Touhami, JA Sahel, JP Berrod, T Léveillard, X Guillonnea, C Delarasse, F Sennlaub
    J Neuroinflammation, 2020-11-26;17(1):358.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Binding of omeprazole to protein targets identified by monoclonal antibodies
    Authors: NMP Cartee, MM Wang
    PLoS ONE, 2020-09-18;15(9):e0239464.
    Species: Mouse
    Sample Types: Reference Standard
  7. Aging-associated changes in CD47 arrangement and interaction with thrombospondin-1 on red blood cells visualized by super-resolution imaging
    Authors: F Wang, YH Liu, T Zhang, J Gao, Y Xu, GY Xie, WJ Zhao, H Wang, YG Yang
    Aging Cell, 2020-08-31;0(0):e13224.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Capture
  8. Embryonic Stem Cell-Derived Peripheral Auditory Neurons Form Neural�Connections with Mouse Central Auditory Neurons In�Vitro via the�?2?1 Receptor
    Authors: Z Liu, Y Jiang, X Li, Z Hu
    Stem Cell Reports, 2018-06-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Thrombospondin-1 signaling through CD47 inhibits cell cycle progression and induces senescence in endothelial cells
    Cell Death Dis, 2016-09-08;7(9):e2368.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Photodynamic therapy-mediated cancer vaccination enhances stem-like phenotype and immune escape, which can be blocked by thrombospondin-1 signaling through CD47 receptor protein.
    Authors: Zheng Y, Zou F, Wang J, Yin G, Le V, Fei Z, Liu J
    J Biol Chem, 2015-02-19;290(14):8975-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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