Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF

Catalog #: 11220-TI Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11220-TI-100

Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Integrin alpha V beta 3 (Catalog # 7889-AV) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse Tie-1 (1-749) Fc Chimera (Catalog # 11220-TI) binds with an ED₅₀ of 2.00-20.0 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Tie-1 protein

Mouse Tie-1 (Ser22-Ala749) Accession # Q06806.3	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q06806.3

N-terminal Sequence
Analysis

Ser22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

106 kDa

SDS-PAGE

105-125 kDa, under reducing conditions.

Product Datasheets

11220-TI

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11220-TI

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Mouse Integrin alpha V beta 3 (7889-AV) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse Tie‑1 (1-749) Fc Chimera (Catalog # 11220-TI) binds with an ED₅₀ of 2.00-20.0 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein (Catalog # 11220-TI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-125 kDa and 210-250 kDa, respectively.

Reconstitution Calculator

Background: Tie-1

Tyrosine -protein kinase with immunoglobulin (Ig) like and epidermal growth factor (EGF) like domains 1 (Tie1), along with the closely related Tie2, is a transmembrane, vascular-specific receptor tyrosine kinase (RTKs) involved in angiogenesis, vascular development, and hematopoiesis (1,2). The Tie molecules are characterized by three Ig-like domains, three EGF-like domains, and three fibronectin type III-like repeats in the extracellular domain (ECD) and a split tyrosine kinase domain in the cytoplasmic region (3,4). The ECD of mouse Tie1 shares 89% amino acid sequence identity with human Tie1. Tie1 is expressed in endothelial and some hematopoietic progenitor cells and helps modulate angiopoietin signaling (5). While Tie1 is unable to directly bind any of the angiopoietins, it can modulate Tie2/angiopoietin signaling through Tie1/Tie2 heterodimer formation (6). Upregulation of Tie1 has been found in some leukemia and tumor related endothelial cells and inhibition of Tie1 has been shown to prevent metastasis and is being studied as a target for anti-angiogenesis treatments (7-9).

References

Thomas, M. and Augustin, H. (2009) Angiogenesis 12:125.
Fagiani, E. and Christofori, G. (2013) Cancer Letters 328:1.
Seegar, T.C.M. et al. (2010). Mol. Cell. 37:643.
Barton, W.A. et al. (2006). Nat Struc & Molec Biol. 13:524.
Saharinen, P. et al. (2017) Nat Rev Drug Discov. 16:635.
Yuan, H.T. et al. (2007) FASEB J. 21:3171.
Yang, P. et al. (2015). J. Huazhong Univ. Sci. Technol. Med. Sci. 35:615-622.
D'Amico, G. et al. (2014) J Clin Invest. 124:824.
Khan, K.A. and Kerbel, R.S. (2020) EMBO molecular medicine 12:e12355.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1

Entrez Gene IDs

7075 (Human); 21846 (Mouse)

Alternate Names

EC 2.7.10; EC 2.7.10.1; JTK14soluble TIE1 variant 3; receptor tyrosine kinase; soluble TIE1 variant 1; soluble TIE1 variant 2; soluble TIE1 variant 5; Tie1; Tie-1; TIEsoluble TIE1 variant 4; tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; tyrosine-protein kinase receptor Tie-1