Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF Summary
Product Specifications
Met | Mouse VEGFR3 (Tyr25-Asp770) Accession # P35917 | IEGRDID | Human IgG1 (Pro100-Lys330) | 6-His tag |
N-terminus | C-terminus |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
743-R3
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: VEGFR3/Flt-4
VEGFR3 (Flt-4), together with VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.
In adults, VEGFR3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGFR3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGFR3 has been reported to serve as the receptors for VEGF-C and VEGF-D.
- Finnerty, H. et al. (1993) Oncogene 8:2293.
- Joukov, V. et al. (1996) EMBO J. 15:290.
- Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA. 95:548.
Citations for Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Blockade of VEGF-C signaling inhibits lymphatic malformations driven by oncogenic PIK3CA mutation
Authors: I Martinez-C, Y Zhang, M Petkova, H Ortsäter, S Sjöberg, SD Castillo, P Brouillard, L Libbrecht, D Saur, M Graupera, K Alitalo, L Boon, M Vikkula, T Mäkinen
Nat Commun, 2020-06-08;11(1):2869.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western Blot Control -
Structural and functional features of central nervous system lymphatic vessels.
Authors: Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, Derecki N, Castle D, Mandell J, Lee K, Harris T, Kipnis J
Nature, 2015-06-01;523(7560):337-41.
Species: Mouse
Sample Types: Whole Tissue
Applications: Antibody Absorption -
Vascular endothelial growth factor c promotes ovarian carcinoma progression through paracrine and autocrine mechanisms.
Authors: Decio A, Taraboletti G, Patton V, Alzani R, Perego P, Fruscio R, Jurgensmeier J, Giavazzi R, Belotti D
Am J Pathol, 2014-02-06;184(4):1050-61.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
CD8+ T cells suppress viral replication in the cornea but contribute to VEGF-C-induced lymphatic vessel genesis.
Authors: Conrady CD, Zheng M, Stone DU
J. Immunol., 2012-05-30;189(1):425-32.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling.
Authors: Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R
Nature, 2012-03-18;484(7392):110-4.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
VEGF-A expression by HSV-1-infected cells drives corneal lymphangiogenesis.
Authors: Wuest TR, Carr DJ
J. Exp. Med., 2009-12-21;207(1):101-15, S1-2.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
VEGF-A produced by chronically inflamed tissue induces lymphangiogenesis in draining lymph nodes.
Authors: Halin C, Tobler NE, Vigl B, Brown LF, Detmar M
Blood, 2007-07-11;110(9):3158-67.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Nonvascular VEGF receptor 3 expression by corneal epithelium maintains avascularity and vision.
Authors: Cursiefen C, Chen L, Saint-Geniez M, Hamrah P, Jin Y, Rashid S, Pytowski B, Persaud K, Wu Y, Streilein JW, Dana R
Proc. Natl. Acad. Sci. U.S.A., 2006-07-18;103(30):11405-10.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Hepatocyte growth factor is a lymphangiogenic factor with an indirect mechanism of action.
Authors: Cao R, Bjorndahl MA, Gallego MI, Chen S, Religa P, Hansen AJ, Cao Y
Blood, 2006-01-19;107(9):3531-6.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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