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Recombinant Mouse Wnt-1/sFRP-1 Complex Protein

Catalog #: 9765-WN Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9765-WN-010
Recombinant Mouse Wnt-1/sFRP-1 Complex Protein Bioactivity
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Recombinant Mouse Wnt-1/sFRP-1 Complex Protein Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.03-0.18 μg/mL
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-1 protein
Mouse Wnt-1
(Ala28-Leu370)
Accession # P04426
Mouse sFRP-1
(Ser32-Lys314)
Accession # Q8C4U3
N-terminus C-terminus
Accession #
P04426, Q8C4U3
N-terminal Sequence
Analysis
Ala28 (Wnt-1) & Ser32, Phe39 (sFRP-1)
Structure / Form
Non-covalent complex
Predicted Molecular Mass
38 kDa (Wnt-1) & 33 kDa (sFRP-1)
SDS-PAGE
35-59 kDa, reducing conditions

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9765-WN

Product Datasheet
COA
SDS

9765-WN

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Wnt-1/sFRP-1 Complex Protein Bioactivity View Larger

Recombinant Mouse Wnt-1/sFRP-1 Complex (Catalog # 9765-WN) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.03-0.18 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-1

Wnt-1 is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins that are important for normal developmental processes (1). Mouse Wnt-1 cDNA encodes a 370 amino acid (aa) protein that contains four potential N-linked glycosylation sites plus 23 cysteines (2, 3). When secreted, it exists as a 343 aa glycoprotein with a molecular weight of 42 to 48 kDa. Mouse Wnt -1 shares a 99.7% aa sequence identity with human and rat Wnt-1. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (4). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (5). Wnt-1 is known to bind to sFRPs, a family of secreted molecules that contain an N-terminal cysteine-rich domain (CRD) highly similar to the CRDs of the Frizzled family receptors (6,7). More than one molecule of sFRP is suggested to participate in the interaction with each Wnt molecule (7). R&D Systems produces biologically active mouse Wnt-1 protein in a complex with mouse sFRP-1, and this Wnt-1/sFRP-1 complex is stable in the physiological buffer without detergent.

References
  1. Willert, K. and R. Nusse (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. Van Ooyen, A. et al. (1985) EMBO J. 4:2905.
  3. Burrus, L.W. and A.P. McMahon (1995) Exp. Cell Res. 220:363.
  4. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  5. Korinek, V. et al. (1997) Science 275:1784.
  6. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  7. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
Long Name
Wingless-type MMTV Integration Site Family, Member 1
Entrez Gene IDs
7471 (Human); 22408 (Mouse)
Alternate Names
Int-1; INT1Proto-oncogene Int-1 homolog; proto-oncogene Wnt-1; wingless-type MMTV integration site family, member 1 (oncogene INT1); wingless-type MMTV integration site family, member 1; Wnt1; Wnt-1

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