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Recombinant Mouse Wnt-10b Protein

Catalog #: 2110-WN
4 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
2110-WN-010/CF

With Carrier

Catalog # Availability Size / Price Qty
2110-WN-010
Recombinant Mouse Wnt-10b Protein Bioactivity
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Product Details
Citations (4)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Wnt-10b Protein Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation/survival assay using IEC‑18 rat small intestinal epithelial cells. The ED50 for this effect is 0.3-1.2 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-10b protein
Met1-Lys389
Accession #
NP_035848
N-terminal Sequence
Analysis
Asn29
Predicted Molecular Mass
40.1 kDa
SDS-PAGE
42 kDa, reducing conditions

Product Datasheets

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2110-WN (with carrier)

Product Datasheet
COA
SDS

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2110-WN/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2110-WN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2110-WN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Wnt-10b Protein Bioactivity View Larger

Recombinant Mouse Wnt-10b (Catalog # 2110-WN) stimulates cell proliferation/survival of the IEC-18 rat small intestinal epithelial cell line. The ED50 for this effect is 0.3-1.2 μg/mL.

SDS-PAGE Recombinant Mouse Wnt-10b Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse Wnt-10b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 42 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-10b

Wnt-10b (also known as Wnt-12) is a 42‑44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Mouse Wnt-10b cDNA encodes a 361 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). A short isoform is reported that lacks aa 143‑238 of the precursor (1, 4). Mouse Wnt-10b shares 97‑98% aa identity with human, rat, equine, porcine and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5‑7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5‑7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a mis‑sense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10 is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14). As a key regulator, Wnt signaling plays a major role in the process of colon carcinogenesis (15).

References
  1. Wang, J. and G.M. Shackleford (1996) Oncogene 13:1537.
  2. Katoh, M. (2008) Curr. Drug Targets 9:565.
  3. Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
  4. Swiss Prot Accession # P48614.
  5. Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
  6. Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
  7. Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
  8. Wright, W.S. et al. (2007) Diabetes 56:295.
  9. Abiola, M. et al. (2009) PLoS ONE 4:e8509.
  10. Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
  11. Christodoulides, C. et al. (2006) Diabetologia 49:678.
  12. Terauchi, M. et al. (2009) Cell Metab. 10:229.
  13. Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
  14. Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.
  15. Cynthia K. et al. (2007) Proc. Natl. Acad. Sci. USA.
Long Name
Wingless-type MMTV Integration Site Family, Member 10B
Entrez Gene IDs
7480 (Human); 22410 (Mouse)
Alternate Names
protein Wnt-10b; Protein Wnt-12; SHFM6WNT-10B protein; wingless-type MMTV integration site family, member 10B; Wnt10b; Wnt-10b; WNT12; Wnt-12

Citations for Recombinant Mouse Wnt-10b Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Impaired differentiation potential of CD34-positive cells derived from mouse hair follicles after long-term culture
    Authors: Y Ouji, M Misu, T Kitamura, D Okuzaki, M Yoshikawa
    Scientific Reports, 2022-06-30;12(1):11011.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Adipogenesis of skeletal muscle fibro/adipogenic progenitors is affected by the WNT5a/GSK3/beta-catenin axis
    Authors: A Reggio, M Rosina, A Palma, A Cerquone P, LL Petrilli, C Gargioli, C Fuoco, E Micarelli, G Giuliani, M Cerretani, A Bresciani, F Sacco, L Castagnoli, G Cesareni
    Cell Death Differ., 2020-05-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets
    Authors: CE Macdougall, EG Wood, J Loschko, V Scagliotti, FC Cassidy, ME Robinson, N Feldhahn, L Castellano, MB Voisin, F Marelli-Be, C Gaston-Mas, M Charalambo, MP Longhi
    Cell Metab., 2018-03-06;27(3):588-601.e4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Noncanonical Wnt5a enhances Wnt/beta-catenin signaling during osteoblastogenesis.
    Authors: Okamoto M, Udagawa N, Uehara S, Maeda K, Yamashita T, Nakamichi Y, Kato H, Saito N, Minami Y, Takahashi N, Kobayashi Y
    Sci Rep, 2014-03-27;4(0):4493.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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